Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
8209 | 5' | -54 | NC_001978.2 | + | 10137 | 0.66 | 0.682001 |
Target: 5'- -gUCaAGCgCGACG--GCGGUAAUCGAa -3' miRNA: 3'- gaAG-UCGaGCUGCgaCGCCGUUAGCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 34691 | 0.66 | 0.670715 |
Target: 5'- -gUCGGCUCGAacccggcaaGCUG-GGCGuucaGUCGGu -3' miRNA: 3'- gaAGUCGAGCUg--------CGACgCCGU----UAGCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 15632 | 0.67 | 0.663924 |
Target: 5'- --aCGGCUCGAcgguuccggcggcuuCGCUGgGGC--UCGAc -3' miRNA: 3'- gaaGUCGAGCU---------------GCGACgCCGuuAGCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 23867 | 0.67 | 0.648039 |
Target: 5'- --cCGGgUCGAgCGCUGCcacuagucGGCAAUCGc -3' miRNA: 3'- gaaGUCgAGCU-GCGACG--------CCGUUAGCu -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 19313 | 0.67 | 0.636672 |
Target: 5'- gCUUCAugGCUucccCGGCGUgcgGCGGCAAcCGGg -3' miRNA: 3'- -GAAGU--CGA----GCUGCGa--CGCCGUUaGCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 4597 | 0.67 | 0.625301 |
Target: 5'- cCUUCcgcguGCUCGACGgUG-GGCAG-CGAc -3' miRNA: 3'- -GAAGu----CGAGCUGCgACgCCGUUaGCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 12352 | 0.68 | 0.591267 |
Target: 5'- --aCGGC-CGgacaACGCgGCGGCAAUCGc -3' miRNA: 3'- gaaGUCGaGC----UGCGaCGCCGUUAGCu -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 9796 | 0.68 | 0.557562 |
Target: 5'- -gUCGGCcguugaGACGCUG-GGCAagGUCGAc -3' miRNA: 3'- gaAGUCGag----CUGCGACgCCGU--UAGCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 8669 | 0.68 | 0.546442 |
Target: 5'- gCUUCAGCUuccCGACGU--CGGCGGgcUCGAu -3' miRNA: 3'- -GAAGUCGA---GCUGCGacGCCGUU--AGCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 32983 | 0.69 | 0.512461 |
Target: 5'- gCUUCAGgCguaCGACGCUGgGGUGAagcuucuUCGAg -3' miRNA: 3'- -GAAGUC-Ga--GCUGCGACgCCGUU-------AGCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 30639 | 0.7 | 0.471016 |
Target: 5'- aCUcCGGCg-GACGUUGCGGCGcUUGAa -3' miRNA: 3'- -GAaGUCGagCUGCGACGCCGUuAGCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 22263 | 0.7 | 0.471016 |
Target: 5'- ---uGGCUCGACGCcGaCGGCAAccCGAc -3' miRNA: 3'- gaagUCGAGCUGCGaC-GCCGUUa-GCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 33371 | 0.71 | 0.391818 |
Target: 5'- gUUCGGUUCG-CGCUGCccaCAAUCGAg -3' miRNA: 3'- gAAGUCGAGCuGCGACGcc-GUUAGCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 16433 | 0.74 | 0.282292 |
Target: 5'- uCUUCGGaaCGGCGCUGggaacguUGGCAAUCGGc -3' miRNA: 3'- -GAAGUCgaGCUGCGAC-------GCCGUUAGCU- -5' |
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8209 | 5' | -54 | NC_001978.2 | + | 33336 | 1.09 | 0.000907 |
Target: 5'- cCUUCAGCUCGACGCUGCGGCAAUCGAc -3' miRNA: 3'- -GAAGUCGAGCUGCGACGCCGUUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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