Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8210 | 3' | -55.2 | NC_001978.2 | + | 18016 | 0.66 | 0.637978 |
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Target: 5'- aGGGugcucCGCGUACcggUC-CGgucCGGGUGCc -3' miRNA: 3'- aCCCu----GCGCAUGaa-AGaGC---GCCCACG- -5' |
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| 8210 | 3' | -55.2 | NC_001978.2 | + | 23990 | 0.66 | 0.637978 |
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Target: 5'- aUGGGaaaGCGCGgaacggucACUgaCUaCGCGGGUGa -3' miRNA: 3'- -ACCC---UGCGCa-------UGAaaGA-GCGCCCACg -5' |
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| 8210 | 3' | -55.2 | NC_001978.2 | + | 34104 | 0.66 | 0.637978 |
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Target: 5'- cUGGGaACGCGUACagUCcgCGCGaGUcGCc -3' miRNA: 3'- -ACCC-UGCGCAUGaaAGa-GCGCcCA-CG- -5' |
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| 8210 | 3' | -55.2 | NC_001978.2 | + | 26305 | 0.66 | 0.6268 |
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Target: 5'- aUGGG-CGCGgcGCUUgugCUCGCcGGUuGCc -3' miRNA: 3'- -ACCCuGCGCa-UGAAa--GAGCGcCCA-CG- -5' |
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| 8210 | 3' | -55.2 | NC_001978.2 | + | 12846 | 0.67 | 0.578912 |
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Target: 5'- cGGGugGCGUcggccguaucgcGCUUaugUCgUCGggcucgauugaguaCGGGUGCg -3' miRNA: 3'- aCCCugCGCA------------UGAA---AG-AGC--------------GCCCACG- -5' |
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| 8210 | 3' | -55.2 | NC_001978.2 | + | 7522 | 0.68 | 0.538346 |
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Target: 5'- -uGGACGCGUacggccguugaGCUUUCccgUGCGGGcaGCg -3' miRNA: 3'- acCCUGCGCA-----------UGAAAGa--GCGCCCa-CG- -5' |
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| 8210 | 3' | -55.2 | NC_001978.2 | + | 6893 | 0.7 | 0.433721 |
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Target: 5'- --cGGCGCGUACagcgCUCuaacgguccguguGCGGGUGCg -3' miRNA: 3'- accCUGCGCAUGaaa-GAG-------------CGCCCACG- -5' |
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| 8210 | 3' | -55.2 | NC_001978.2 | + | 39441 | 0.71 | 0.369415 |
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Target: 5'- gGGGGCGCGgcaagggGCUUUCcCG-GGG-GCa -3' miRNA: 3'- aCCCUGCGCa------UGAAAGaGCgCCCaCG- -5' |
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| 8210 | 3' | -55.2 | NC_001978.2 | + | 33997 | 1.11 | 0.00051 |
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Target: 5'- cUGGGACGCGUACUUUCUCGCGGGUGCu -3' miRNA: 3'- -ACCCUGCGCAUGAAAGAGCGCCCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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