Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8213 | 3' | -57.5 | NC_001978.2 | + | 26863 | 0.66 | 0.472959 |
Target: 5'- gGCaAGGUCguUGGGAAuGCCCCcGCCg- -3' miRNA: 3'- gCGaUUCGG--ACCCUU-CGGGGaUGGau -5' |
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8213 | 3' | -57.5 | NC_001978.2 | + | 39515 | 0.68 | 0.36694 |
Target: 5'- gGCUu-GCCcccGGGaAAGCCCUUGCCg- -3' miRNA: 3'- gCGAuuCGGa--CCC-UUCGGGGAUGGau -5' |
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8213 | 3' | -57.5 | NC_001978.2 | + | 25498 | 0.68 | 0.366053 |
Target: 5'- gCGCcucAGCCUGGGcgacgucGAGCCCgaACCg- -3' miRNA: 3'- -GCGau-UCGGACCC-------UUCGGGgaUGGau -5' |
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8213 | 3' | -57.5 | NC_001978.2 | + | 41246 | 0.68 | 0.358143 |
Target: 5'- uGCUAccuGCCUGGGuuGGUaCCUGCCa- -3' miRNA: 3'- gCGAUu--CGGACCCu-UCGgGGAUGGau -5' |
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8213 | 3' | -57.5 | NC_001978.2 | + | 9043 | 0.68 | 0.349496 |
Target: 5'- cCGC--AGCaaGGGcAAGCCCCUucGCCUGu -3' miRNA: 3'- -GCGauUCGgaCCC-UUCGGGGA--UGGAU- -5' |
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8213 | 3' | -57.5 | NC_001978.2 | + | 2976 | 0.68 | 0.332651 |
Target: 5'- cCGUcGAGCgCUGGGGAguGCCCCcGCCc- -3' miRNA: 3'- -GCGaUUCG-GACCCUU--CGGGGaUGGau -5' |
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8213 | 3' | -57.5 | NC_001978.2 | + | 24898 | 0.69 | 0.324455 |
Target: 5'- cCGCUGAGCaaGGGggGCgCCgACgCUGc -3' miRNA: 3'- -GCGAUUCGgaCCCuuCGgGGaUG-GAU- -5' |
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8213 | 3' | -57.5 | NC_001978.2 | + | 32662 | 0.69 | 0.31641 |
Target: 5'- aCGCUGAcGCCcuggGGGAAGCCgCCggGCgUAu -3' miRNA: 3'- -GCGAUU-CGGa---CCCUUCGG-GGa-UGgAU- -5' |
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8213 | 3' | -57.5 | NC_001978.2 | + | 36652 | 0.71 | 0.213537 |
Target: 5'- aCGCUGAGC--GGGAAGCCCUgagcGCCc- -3' miRNA: 3'- -GCGAUUCGgaCCCUUCGGGGa---UGGau -5' |
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8213 | 3' | -57.5 | NC_001978.2 | + | 108 | 0.72 | 0.200573 |
Target: 5'- uCGUU-GGCCUGGGggGCgCCUggguccggcggcucACCUAg -3' miRNA: 3'- -GCGAuUCGGACCCuuCGgGGA--------------UGGAU- -5' |
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8213 | 3' | -57.5 | NC_001978.2 | + | 34724 | 1.06 | 0.000507 |
Target: 5'- gCGCUAAGCCUGGGAAGCCCCUACCUAc -3' miRNA: 3'- -GCGAUUCGGACCCUUCGGGGAUGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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