miRNA: 3'- -UCAUCCAUCCCCGAA--------------AAa----GCACaaGu -5'
miRNA: 3'- -UCAuCCA-----UCCCCGAAAaAGCACAAGu -5'
miRNA: 3'- -UCAUCCAUCCCCGAAAAAGCACAAGU- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8213 | 5' | -48.3 | NC_001978.2 | + | 34793 | 0.7 | 0.798967 |
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Target: 5'- gAGUAGGUAGGGGCUUcccaggcuuagcgcUUgaagaUGUGggCu -3' miRNA: 3'- -UCAUCCAUCCCCGAA--------------AAa----GCACaaGu -5' |
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| 8213 | 5' | -48.3 | NC_001978.2 | + | 19121 | 0.74 | 0.560232 |
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Target: 5'- gGGUuGGUccggcGGGGGCUUUcUUGUGUUCc -3' miRNA: 3'- -UCAuCCA-----UCCCCGAAAaAGCACAAGu -5' |
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| 8213 | 5' | -48.3 | NC_001978.2 | + | 34758 | 1.1 | 0.002862 |
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Target: 5'- gAGUAGGUAGGGGCUUUUUCGUGUUCAa -3' miRNA: 3'- -UCAUCCAUCCCCGAAAAAGCACAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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