Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8215 | 5' | -54.4 | NC_001978.2 | + | 10301 | 0.66 | 0.707087 |
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Target: 5'- uGGCGGCGGCG--UGGcUUCUcgcuaugGGUCCg- -3' miRNA: 3'- -CCGCCGUUGCcaACC-AAGA-------UCAGGgc -5' |
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| 8215 | 5' | -54.4 | NC_001978.2 | + | 13288 | 0.67 | 0.652701 |
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Target: 5'- cGGCGGCAA-GGUcGGccgUCgUGGUCCa- -3' miRNA: 3'- -CCGCCGUUgCCAaCCa--AG-AUCAGGgc -5' |
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| 8215 | 5' | -54.4 | NC_001978.2 | + | 40278 | 0.68 | 0.607887 |
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Target: 5'- uGgGGCAGCGGgaagGGUcggCcGGUCCCc -3' miRNA: 3'- cCgCCGUUGCCaa--CCAa--GaUCAGGGc -5' |
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| 8215 | 5' | -54.4 | NC_001978.2 | + | 1600 | 0.7 | 0.477679 |
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Target: 5'- cGGCGGCAAUGGUgc--UCU--UCCCGu -3' miRNA: 3'- -CCGCCGUUGCCAaccaAGAucAGGGC- -5' |
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| 8215 | 5' | -54.4 | NC_001978.2 | + | 19335 | 0.72 | 0.362727 |
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Target: 5'- cGGCGGCAAcCGGgcaucGGagcgCUAcGUCCCGa -3' miRNA: 3'- -CCGCCGUU-GCCaa---CCaa--GAU-CAGGGC- -5' |
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| 8215 | 5' | -54.4 | NC_001978.2 | + | 36161 | 1.12 | 0.000552 |
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Target: 5'- cGGCGGCAACGGUUGGUUCUAGUCCCGg -3' miRNA: 3'- -CCGCCGUUGCCAACCAAGAUCAGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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