Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8217 | 5' | -59.3 | NC_001978.2 | + | 37765 | 1.11 | 0.000199 |
Target: 5'- aCGGCGACCCCUCAACCCUCAUGCCGCu -3' miRNA: 3'- -GCCGCUGGGGAGUUGGGAGUACGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 32480 | 0.85 | 0.019953 |
Target: 5'- aGGCGuaaGCCCCUCAGCCCagUCGUGCgGCu -3' miRNA: 3'- gCCGC---UGGGGAGUUGGG--AGUACGgCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 14870 | 0.76 | 0.094813 |
Target: 5'- uCGGCG-UCCCg-AACCUUCAUGCCGUu -3' miRNA: 3'- -GCCGCuGGGGagUUGGGAGUACGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 2024 | 0.74 | 0.121914 |
Target: 5'- aCGGuCGACCaCCUUGACCCggaCGUGUgGCg -3' miRNA: 3'- -GCC-GCUGG-GGAGUUGGGa--GUACGgCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 1906 | 0.74 | 0.132447 |
Target: 5'- cCGGaccCGACCCCgacgGACCCUU-UGCCGCa -3' miRNA: 3'- -GCC---GCUGGGGag--UUGGGAGuACGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 20304 | 0.74 | 0.13993 |
Target: 5'- uCGGCGGCUuccuucacggCCUCAACCgUCucgGCCGUc -3' miRNA: 3'- -GCCGCUGG----------GGAGUUGGgAGua-CGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 29850 | 0.72 | 0.188388 |
Target: 5'- gGGCGACCCUUCcggguGCCgaCUCcUGCCGa -3' miRNA: 3'- gCCGCUGGGGAGu----UGG--GAGuACGGCg -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 9664 | 0.71 | 0.214957 |
Target: 5'- uCGGCG-CCgUUCAGCCC-CAUggacuuguacGCCGCg -3' miRNA: 3'- -GCCGCuGGgGAGUUGGGaGUA----------CGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 29017 | 0.71 | 0.214957 |
Target: 5'- cCGGCG-CCCUUgAacACCUUCcgGUCGCg -3' miRNA: 3'- -GCCGCuGGGGAgU--UGGGAGuaCGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 17921 | 0.7 | 0.226455 |
Target: 5'- -aGCGACCCUUC-GCCCggCAcGCCGUc -3' miRNA: 3'- gcCGCUGGGGAGuUGGGa-GUaCGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 7401 | 0.7 | 0.256834 |
Target: 5'- aGGCGACCUUcgagccgUCGACCC-CggGCCGa -3' miRNA: 3'- gCCGCUGGGG-------AGUUGGGaGuaCGGCg -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 16062 | 0.69 | 0.299215 |
Target: 5'- cCGGcCGGaCCCUCcgGACCCUCcgGCCc- -3' miRNA: 3'- -GCC-GCUgGGGAG--UUGGGAGuaCGGcg -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 26105 | 0.69 | 0.299215 |
Target: 5'- aCGGUGcuCCgCCUCAACCUgggCAcgGUCGCg -3' miRNA: 3'- -GCCGCu-GG-GGAGUUGGGa--GUa-CGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 15810 | 0.68 | 0.321968 |
Target: 5'- gGGC-ACCCCgUCGGCCgaCGUGcCCGUu -3' miRNA: 3'- gCCGcUGGGG-AGUUGGgaGUAC-GGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 28075 | 0.68 | 0.321968 |
Target: 5'- gGGCGACCUaacggCggUCUUCcgGUCGCu -3' miRNA: 3'- gCCGCUGGGga---GuuGGGAGuaCGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 39528 | 0.68 | 0.327459 |
Target: 5'- uGGCGGacagaauggcuugcCCCCgggaaAGCCCU--UGCCGCg -3' miRNA: 3'- gCCGCU--------------GGGGag---UUGGGAguACGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 32285 | 0.68 | 0.314243 |
Target: 5'- uGGCGcgcuGCCgCUCAG-CCUCA-GCCGUg -3' miRNA: 3'- gCCGC----UGGgGAGUUgGGAGUaCGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 1408 | 0.68 | 0.306659 |
Target: 5'- -cGCGACCCggUAACCCUCAcugggaaccGUCGCg -3' miRNA: 3'- gcCGCUGGGgaGUUGGGAGUa--------CGGCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 24872 | 0.68 | 0.345989 |
Target: 5'- aGGCGACCCauCUUGACCa-CGUGCaccCGCu -3' miRNA: 3'- gCCGCUGGG--GAGUUGGgaGUACG---GCG- -5' |
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8217 | 5' | -59.3 | NC_001978.2 | + | 29206 | 0.68 | 0.349287 |
Target: 5'- aGGCgccGACCCgUgAcggacacagugacaaGCCC-CGUGCCGCg -3' miRNA: 3'- gCCG---CUGGGgAgU---------------UGGGaGUACGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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