Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8218 | 5' | -48.1 | NC_001978.2 | + | 5673 | 0.67 | 0.939171 |
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Target: 5'- --cCGGuCGCGUCCguca-GGAUGCCg -3' miRNA: 3'- caaGCUuGCGUAGGacuugCUUACGG- -5' |
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| 8218 | 5' | -48.1 | NC_001978.2 | + | 24635 | 0.67 | 0.939171 |
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Target: 5'- --gUGcGCGCA-CCgUGAGCGggUGCg -3' miRNA: 3'- caaGCuUGCGUaGG-ACUUGCuuACGg -5' |
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| 8218 | 5' | -48.1 | NC_001978.2 | + | 22407 | 0.66 | 0.958508 |
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Target: 5'- --cCGGACgGCAaCCggGAACcGAUGCCg -3' miRNA: 3'- caaGCUUG-CGUaGGa-CUUGcUUACGG- -5' |
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| 8218 | 5' | -48.1 | NC_001978.2 | + | 6480 | 0.66 | 0.960175 |
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Target: 5'- -gUCGGGCGCcaauuucccccagCCUGGGCaagcuGAAUGCUg -3' miRNA: 3'- caAGCUUGCGua-----------GGACUUG-----CUUACGG- -5' |
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| 8218 | 5' | -48.1 | NC_001978.2 | + | 33110 | 0.66 | 0.966382 |
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Target: 5'- cUUCGGGCGUggcgugacGUCCUGGgcaagcguuggcACGu-UGCCg -3' miRNA: 3'- cAAGCUUGCG--------UAGGACU------------UGCuuACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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