Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8219 | 3' | -48.5 | NC_001978.2 | + | 17328 | 0.66 | 0.961522 |
Target: 5'- cCGGCG-GUCgGCGCucAGGuguUCUUCGg -3' miRNA: 3'- aGCUGCgCAG-CGUGuuUCUu--AGAAGC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 33061 | 0.66 | 0.957825 |
Target: 5'- cUCGAagaagcuucaccccaGCGUCGUACGccuGAAgcgCUUCGg -3' miRNA: 3'- -AGCUg--------------CGCAGCGUGUuu-CUUa--GAAGC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 20414 | 0.66 | 0.9574 |
Target: 5'- gUCGGcCGaCGUCGC-CGAAGA--CUUCa -3' miRNA: 3'- -AGCU-GC-GCAGCGuGUUUCUuaGAAGc -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 28641 | 0.66 | 0.952987 |
Target: 5'- cCG-CGCGcuUCGCuCGAAGGAcggCUUCGg -3' miRNA: 3'- aGCuGCGC--AGCGuGUUUCUUa--GAAGC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 19480 | 0.66 | 0.950196 |
Target: 5'- -aGGCGCucuUCGCGCuccgccucagcggcAAGGAUCUUCGc -3' miRNA: 3'- agCUGCGc--AGCGUGu-------------UUCUUAGAAGC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 13819 | 0.66 | 0.948276 |
Target: 5'- gUCGACuucaCGUCGCugAAGGAcugcacgacGUgCUUCGu -3' miRNA: 3'- -AGCUGc---GCAGCGugUUUCU---------UA-GAAGC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 9769 | 0.66 | 0.948276 |
Target: 5'- -aGACGCGUUGCugAAGcucGAcccGUCgUCGg -3' miRNA: 3'- agCUGCGCAGCGugUUU---CU---UAGaAGC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 25289 | 0.67 | 0.926372 |
Target: 5'- uUCaACGCGUCGgGCGAAG-GUCUgacCGu -3' miRNA: 3'- -AGcUGCGCAGCgUGUUUCuUAGAa--GC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 37461 | 0.67 | 0.926372 |
Target: 5'- aCGACGC-UCGCGCAcauucUCUUCa -3' miRNA: 3'- aGCUGCGcAGCGUGUuucuuAGAAGc -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 28848 | 0.68 | 0.91356 |
Target: 5'- cCGACGUGaagCGCAUGAAGGAcggaUUCGu -3' miRNA: 3'- aGCUGCGCa--GCGUGUUUCUUag--AAGC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 2715 | 0.68 | 0.911531 |
Target: 5'- aCGACGCGUguuccuucgugaugCGCGCGccccGGGcGUCUUCc -3' miRNA: 3'- aGCUGCGCA--------------GCGUGU----UUCuUAGAAGc -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 6342 | 0.68 | 0.90669 |
Target: 5'- cUCGAccaCGUGUCGCGCAuucccgaucGGGcgCUUCa -3' miRNA: 3'- -AGCU---GCGCAGCGUGUu--------UCUuaGAAGc -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 35804 | 0.68 | 0.899514 |
Target: 5'- gCGACgGCGUUGC-CAAGGg--CUUCGc -3' miRNA: 3'- aGCUG-CGCAGCGuGUUUCuuaGAAGC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 17808 | 0.69 | 0.876198 |
Target: 5'- cUUGuACGCGUCGC-CGAAGcc-CUUCGc -3' miRNA: 3'- -AGC-UGCGCAGCGuGUUUCuuaGAAGC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 26431 | 0.7 | 0.831791 |
Target: 5'- uUCGACGCGaacgCGCACcuguuGAG-CUUCGc -3' miRNA: 3'- -AGCUGCGCa---GCGUGuuu--CUUaGAAGC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 27113 | 0.7 | 0.822159 |
Target: 5'- cCGGCGCGaaguagCGCACGAacGGAAUgagCUUCa -3' miRNA: 3'- aGCUGCGCa-----GCGUGUU--UCUUA---GAAGc -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 17857 | 0.7 | 0.822159 |
Target: 5'- aCGGCGUGcCGgGCGAAGggUCg--- -3' miRNA: 3'- aGCUGCGCaGCgUGUUUCuuAGaagc -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 23578 | 0.71 | 0.738069 |
Target: 5'- uUCGGCGCGUCGUACuc-----CUUCGc -3' miRNA: 3'- -AGCUGCGCAGCGUGuuucuuaGAAGC- -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 31774 | 0.74 | 0.622953 |
Target: 5'- uUCaGCGCGUCGCGCGAAucGAAUCcgCa -3' miRNA: 3'- -AGcUGCGCAGCGUGUUU--CUUAGaaGc -5' |
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8219 | 3' | -48.5 | NC_001978.2 | + | 38823 | 1.1 | 0.003771 |
Target: 5'- cUCGACGCGUCGCACAAAGAAUCUUCGc -3' miRNA: 3'- -AGCUGCGCAGCGUGUUUCUUAGAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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