Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8224 | 3' | -54.5 | NC_001978.2 | + | 34176 | 0.69 | 0.507479 |
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Target: 5'- uGAGCG-CCGUCGAGu-GCGCGCc--- -3' miRNA: 3'- -CUUGCgGGUAGCUCuuCGCGCGaagc -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 36349 | 0.67 | 0.584478 |
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Target: 5'- cGACGCCCuUCGuc-AGCGCGCacagCGa -3' miRNA: 3'- cUUGCGGGuAGCucuUCGCGCGaa--GC- -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 37815 | 0.66 | 0.685908 |
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Target: 5'- gGGGuCGCCguCGUCGGGcAGCGCGCa--- -3' miRNA: 3'- -CUU-GCGG--GUAGCUCuUCGCGCGaagc -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 38754 | 0.68 | 0.540044 |
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Target: 5'- -cGCGUCCAUgaccuucaggcgCGAGAAGCGCGacacgUCa -3' miRNA: 3'- cuUGCGGGUA------------GCUCUUCGCGCga---AGc -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 40015 | 0.66 | 0.697058 |
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Target: 5'- ---gGCCCuaaGUCGugguGGggGCGCGCgUCa -3' miRNA: 3'- cuugCGGG---UAGC----UCuuCGCGCGaAGc -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 40123 | 1.09 | 0.000819 |
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Target: 5'- gGAACGCCCAUCGAGAAGCGCGCUUCGa -3' miRNA: 3'- -CUUGCGGGUAGCUCUUCGCGCGAAGC- -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 40203 | 0.75 | 0.20319 |
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Target: 5'- --uCGCCCAcgUGAucGAAGCGCGCUUCu -3' miRNA: 3'- cuuGCGGGUa-GCU--CUUCGCGCGAAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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