Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8228 | 5' | -52.5 | NC_001981.1 | + | 2987 | 0.68 | 0.138825 |
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Target: 5'- uCGCUUGGuguAGCgGGACAACAGCGUCu-- -3' miRNA: 3'- -GCGGGUU---UCG-CUUGUUGUCGCAGcua -5' |
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| 8228 | 5' | -52.5 | NC_001981.1 | + | 4653 | 0.68 | 0.125253 |
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Target: 5'- uGCCgAAGGUGAugucGCAGCGGCGacagUCGGc -3' miRNA: 3'- gCGGgUUUCGCU----UGUUGUCGC----AGCUa -5' |
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| 8228 | 5' | -52.5 | NC_001981.1 | + | 3889 | 0.68 | 0.125253 |
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Target: 5'- gCGCCCAaauaucuugaGAGCGcuCcuGACAGgGUCGAg -3' miRNA: 3'- -GCGGGU----------UUCGCuuG--UUGUCgCAGCUa -5' |
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| 8228 | 5' | -52.5 | NC_001981.1 | + | 488 | 0.7 | 0.091589 |
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Target: 5'- uGCCguuGGCGAGCAGugaGGCGUCGGc -3' miRNA: 3'- gCGGguuUCGCUUGUUg--UCGCAGCUa -5' |
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| 8228 | 5' | -52.5 | NC_001981.1 | + | 3765 | 0.75 | 0.03619 |
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Target: 5'- uCGCCCAu-GUGAagGCGGCuAGCGUCGAa -3' miRNA: 3'- -GCGGGUuuCGCU--UGUUG-UCGCAGCUa -5' |
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| 8228 | 5' | -52.5 | NC_001981.1 | + | 4063 | 1.07 | 0.000081 |
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Target: 5'- gCGCCCAAAGCGAACAACAGCGUCGAUg -3' miRNA: 3'- -GCGGGUUUCGCUUGUUGUCGCAGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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