Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8238 | 3' | -57.3 | NC_001981.1 | + | 1508 | 0.66 | 0.093883 |
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Target: 5'- cUCGCuCGgagacauaauCUGCGACGUCcGCCUguccccugaacaggUCGGCu -3' miRNA: 3'- -AGCG-GC----------GACGCUGUAG-UGGA--------------AGCCGu -5' |
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| 8238 | 3' | -57.3 | NC_001981.1 | + | 71 | 0.66 | 0.085684 |
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Target: 5'- aCGCCGCgGCaGAUGUCGCCUaC-GCGa -3' miRNA: 3'- aGCGGCGaCG-CUGUAGUGGAaGcCGU- -5' |
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| 8238 | 3' | -57.3 | NC_001981.1 | + | 4655 | 0.67 | 0.077374 |
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Target: 5'- gUUGCCGaagGUGAUGUCGCag-CGGCGa -3' miRNA: 3'- -AGCGGCga-CGCUGUAGUGgaaGCCGU- -5' |
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| 8238 | 3' | -57.3 | NC_001981.1 | + | 106 | 0.7 | 0.038829 |
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Target: 5'- aCGuUCGCguagGCGACAUCugCcgCGGCGu -3' miRNA: 3'- aGC-GGCGa---CGCUGUAGugGaaGCCGU- -5' |
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| 8238 | 3' | -57.3 | NC_001981.1 | + | 2198 | 0.73 | 0.024658 |
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Target: 5'- aUGCCGCUcguucGCGACGUCguuaagcaguGCCgUCGGCGc -3' miRNA: 3'- aGCGGCGA-----CGCUGUAG----------UGGaAGCCGU- -5' |
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| 8238 | 3' | -57.3 | NC_001981.1 | + | 4623 | 1.09 | 0.000016 |
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Target: 5'- gUCGCCGCUGCGACAUCACCUUCGGCAa -3' miRNA: 3'- -AGCGGCGACGCUGUAGUGGAAGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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