miRNA: 3'- -GUCGUCGGUGgAUACGuUGGc-GGUGU- -5'
miRNA: 3'- -GUCGUCGGUGGAUACGUUGGCGGUGU- -5'
miRNA: 3'- -GUCGUCGGUGGAUACGUUgGCGGUGU- -5'
miRNA: 3'- -GUCGUCGGUGGAUACGUUGGCGGUGU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8245 | 5' | -56.2 | NC_001987.1 | + | 25864 | 0.75 | 0.294643 |
|
Target: 5'- gCAGCAGCCACgUGUGCuACCcuCUACAg -3' miRNA: 3'- -GUCGUCGGUGgAUACGuUGGc-GGUGU- -5' |
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| 8245 | 5' | -56.2 | NC_001987.1 | + | 25894 | 1.08 | 0.001756 |
|
Target: 5'- gCAGCAGCCACCUAUGCAACCGCCACAu -3' miRNA: 3'- -GUCGUCGGUGGAUACGUUGGCGGUGU- -5' |
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| 8245 | 5' | -56.2 | NC_001987.1 | + | 25924 | 1 | 0.006437 |
|
Target: 5'- gCAGCAGCCACCUAUGCAAgCGCCACAu -3' miRNA: 3'- -GUCGUCGGUGGAUACGUUgGCGGUGU- -5' |
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| 8245 | 5' | -56.2 | NC_001987.1 | + | 25954 | 1 | 0.006819 |
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Target: 5'- uCAGCAGCUACCUAUGCAGCCGCUACAu -3' miRNA: 3'- -GUCGUCGGUGGAUACGUUGGCGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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