Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8280 | 5' | -52 | NC_002031.1 | + | 6780 | 0.66 | 0.392592 |
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Target: 5'- -uGGUUGU-GAUCCC-CGAGCCAGg- -3' miRNA: 3'- cuUCAACAgUUGGGGuGUUCGGUCgg -5' |
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| 8280 | 5' | -52 | NC_002031.1 | + | 5065 | 0.66 | 0.392592 |
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Target: 5'- aGGAGUUGUCA--CCgACAAggauGCCAuuGCCg -3' miRNA: 3'- -CUUCAACAGUugGGgUGUU----CGGU--CGG- -5' |
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| 8280 | 5' | -52 | NC_002031.1 | + | 8170 | 0.67 | 0.314171 |
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Target: 5'- uGAAGgUGUUAGCUCCAUAcauGCCAGa- -3' miRNA: 3'- -CUUCaACAGUUGGGGUGUu--CGGUCgg -5' |
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| 8280 | 5' | -52 | NC_002031.1 | + | 4514 | 0.74 | 0.112408 |
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Target: 5'- gGAGGg---CAGCCCCAaccaAGGCCAGCg -3' miRNA: 3'- -CUUCaacaGUUGGGGUg---UUCGGUCGg -5' |
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| 8280 | 5' | -52 | NC_002031.1 | + | 10520 | 0.78 | 0.052042 |
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Target: 5'- aGAAGUUGUCAGCCCagaaccccaCACGAGUUuuGCCa -3' miRNA: 3'- -CUUCAACAGUUGGG---------GUGUUCGGu-CGG- -5' |
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| 8280 | 5' | -52 | NC_002031.1 | + | 8176 | 1.13 | 0.00009 |
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Target: 5'- aGAAGUUGUCAACCCCACAAGCCAGCCa -3' miRNA: 3'- -CUUCAACAGUUGGGGUGUUCGGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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