Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 831 | 3' | -50.3 | NC_000883.1 | + | 1210 | 0.66 | 0.307878 |
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Target: 5'- uGCCGcuAGAcuCCCCaGCAUcACUACuAGUg -3' miRNA: 3'- -CGGU--UCUuuGGGG-CGUAaUGGUG-UCG- -5' |
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| 831 | 3' | -50.3 | NC_000883.1 | + | 308 | 0.69 | 0.178134 |
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Target: 5'- cGCCAucuuguaccGGAAGUCCCGCc-UACCgGCGGCg -3' miRNA: 3'- -CGGU---------UCUUUGGGGCGuaAUGG-UGUCG- -5' |
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| 831 | 3' | -50.3 | NC_000883.1 | + | 5533 | 0.69 | 0.178134 |
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Target: 5'- cGCCAucuuguaccGGAAGUCCCGCc-UACCgGCGGCg -3' miRNA: 3'- -CGGU---------UCUUUGGGGCGuaAUGG-UGUCG- -5' |
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| 831 | 3' | -50.3 | NC_000883.1 | + | 5287 | 0.69 | 0.178134 |
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Target: 5'- cGCCAucuuguaccGGAAGUCCCGCc-UACCgGCGGCg -3' miRNA: 3'- -CGGU---------UCUUUGGGGCGuaAUGG-UGUCG- -5' |
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| 831 | 3' | -50.3 | NC_000883.1 | + | 62 | 0.69 | 0.178134 |
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Target: 5'- cGCCAucuuguaccGGAAGUCCCGCc-UACCgGCGGCg -3' miRNA: 3'- -CGGU---------UCUUUGGGGCGuaAUGG-UGUCG- -5' |
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| 831 | 3' | -50.3 | NC_000883.1 | + | 1138 | 1.14 | 0.000049 |
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Target: 5'- uGCCAAGAAACCCCGCAUUACCACAGCc -3' miRNA: 3'- -CGGUUCUUUGGGGCGUAAUGGUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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