Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8318 | 5' | -59.5 | NC_002067.1 | + | 22672 | 0.66 | 0.352596 |
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Target: 5'- cGCCUGcacGCCCcacaGCCCg-CGCAGGUu -3' miRNA: 3'- aCGGAC---UGGGua--CGGGagGUGUCCAc -5' |
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| 8318 | 5' | -59.5 | NC_002067.1 | + | 10338 | 0.66 | 0.352596 |
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Target: 5'- cGUCUcGACCCGaGCCCgauagCCGcCAGGa- -3' miRNA: 3'- aCGGA-CUGGGUaCGGGa----GGU-GUCCac -5' |
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| 8318 | 5' | -59.5 | NC_002067.1 | + | 17304 | 0.66 | 0.335759 |
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Target: 5'- aGCCgucugGACCCGcgGCCgC-CCAC-GGUGg -3' miRNA: 3'- aCGGa----CUGGGUa-CGG-GaGGUGuCCAC- -5' |
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| 8318 | 5' | -59.5 | NC_002067.1 | + | 18275 | 0.67 | 0.28882 |
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Target: 5'- cUGCCUG--CCAUGCCuCUCCugcgGCGGGa- -3' miRNA: 3'- -ACGGACugGGUACGG-GAGG----UGUCCac -5' |
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| 8318 | 5' | -59.5 | NC_002067.1 | + | 8762 | 0.67 | 0.281517 |
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Target: 5'- gGUgaGACUCggugGUGCCCUCUAC-GGUGa -3' miRNA: 3'- aCGgaCUGGG----UACGGGAGGUGuCCAC- -5' |
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| 8318 | 5' | -59.5 | NC_002067.1 | + | 25498 | 0.72 | 0.139168 |
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Target: 5'- gGCCUGGCCgCggGCgCCUCC-CAGGa- -3' miRNA: 3'- aCGGACUGG-GuaCG-GGAGGuGUCCac -5' |
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| 8318 | 5' | -59.5 | NC_002067.1 | + | 8638 | 0.72 | 0.135293 |
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Target: 5'- cGCCgcGACCCGcGCgCUCCAC-GGUGg -3' miRNA: 3'- aCGGa-CUGGGUaCGgGAGGUGuCCAC- -5' |
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| 8318 | 5' | -59.5 | NC_002067.1 | + | 16078 | 0.72 | 0.127838 |
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Target: 5'- aGCCUGaaGCCCGUGaCCUgCAgCAGGUGc -3' miRNA: 3'- aCGGAC--UGGGUACgGGAgGU-GUCCAC- -5' |
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| 8318 | 5' | -59.5 | NC_002067.1 | + | 22516 | 0.77 | 0.05906 |
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Target: 5'- cGCCUGGCCUAUGgCCUCgcgguacagcguuaGCAGGUGa -3' miRNA: 3'- aCGGACUGGGUACgGGAGg-------------UGUCCAC- -5' |
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| 8318 | 5' | -59.5 | NC_002067.1 | + | 33113 | 1.07 | 0.000251 |
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Target: 5'- cUGCCUGACCCAUGCCCUCCACAGGUGc -3' miRNA: 3'- -ACGGACUGGGUACGGGAGGUGUCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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