Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8321 | 3' | -51.5 | NC_002067.1 | + | 5326 | 0.67 | 0.75182 |
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Target: 5'- aGGAggGAuUGCAgGGcguAGAGCUUGGGCGc -3' miRNA: 3'- -CCUa-CUcACGUaCC---UUUUGGACCCGC- -5' |
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| 8321 | 3' | -51.5 | NC_002067.1 | + | 2293 | 0.67 | 0.729588 |
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Target: 5'- gGGAUGAGUugggccugauGCA-GGAuaaauauGGCCUGGaGCa -3' miRNA: 3'- -CCUACUCA----------CGUaCCUu------UUGGACC-CGc -5' |
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| 8321 | 3' | -51.5 | NC_002067.1 | + | 7247 | 0.68 | 0.669999 |
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Target: 5'- cGGGUGA-UGCGgaaagggcccgggacGGAGgcucgguuguugauGACCUGGGCGg -3' miRNA: 3'- -CCUACUcACGUa--------------CCUU--------------UUGGACCCGC- -5' |
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| 8321 | 3' | -51.5 | NC_002067.1 | + | 19656 | 0.69 | 0.625657 |
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Target: 5'- uGGcgGGcGUGCuccgaAUGGc--GCCUGGGCGa -3' miRNA: 3'- -CCuaCU-CACG-----UACCuuuUGGACCCGC- -5' |
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| 8321 | 3' | -51.5 | NC_002067.1 | + | 10430 | 0.71 | 0.522042 |
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Target: 5'- uGGAUGGcGUGCAauUGGgcGAggaucgcgcCCUGGGUGa -3' miRNA: 3'- -CCUACU-CACGU--ACCuuUU---------GGACCCGC- -5' |
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| 8321 | 3' | -51.5 | NC_002067.1 | + | 30672 | 1.13 | 0.000772 |
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Target: 5'- uGGAUGAGUGCAUGGAAAACCUGGGCGg -3' miRNA: 3'- -CCUACUCACGUACCUUUUGGACCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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