Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8331 | 5' | -54.6 | NC_002067.1 | + | 15505 | 0.66 | 0.657502 |
Target: 5'- aCGGAGgcgCCgUCGaCGCGCAGguCGUu -3' miRNA: 3'- aGCUUUa--GG-AGCaGCGCGUCguGCGc -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 4707 | 0.66 | 0.634812 |
Target: 5'- aUCGAGGagCUCcUUG-GCGGCACGCu -3' miRNA: 3'- -AGCUUUagGAGcAGCgCGUCGUGCGc -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 25336 | 0.66 | 0.627999 |
Target: 5'- cCGAGcgCgUCGUCGCcgaccuggcgcucguGCAGC-CGCu -3' miRNA: 3'- aGCUUuaGgAGCAGCG---------------CGUCGuGCGc -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 23306 | 0.67 | 0.605312 |
Target: 5'- uUCGAAGggCUCGgucgggacgagacgCGCGCGGCgaaACGCu -3' miRNA: 3'- -AGCUUUagGAGCa-------------GCGCGUCG---UGCGc -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 5256 | 0.67 | 0.589485 |
Target: 5'- gUUGAGggCCUCGgcgGCGUGGCccuugGCGCGg -3' miRNA: 3'- -AGCUUuaGGAGCag-CGCGUCG-----UGCGC- -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 23449 | 0.67 | 0.589485 |
Target: 5'- gUCGAAGuangacUCCUCGcCGC-CGGCgGCGCc -3' miRNA: 3'- -AGCUUU------AGGAGCaGCGcGUCG-UGCGc -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 3600 | 0.67 | 0.586102 |
Target: 5'- gUGggGaaCUCGUCGCuugacagcaccgccGCAGC-CGCGg -3' miRNA: 3'- aGCuuUagGAGCAGCG--------------CGUCGuGCGC- -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 8745 | 0.67 | 0.555854 |
Target: 5'- aCGuccccGUCggCGUCGCGC-GCGCGCa -3' miRNA: 3'- aGCuu---UAGgaGCAGCGCGuCGUGCGc -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 15408 | 0.67 | 0.544761 |
Target: 5'- gUCGuuggUgUCGUUGCGCAGCAUGg- -3' miRNA: 3'- -AGCuuuaGgAGCAGCGCGUCGUGCgc -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 31388 | 0.68 | 0.532643 |
Target: 5'- cUCGAGGccagUCUCGUCGCugucauggcggcuGCGGCugcCGCGg -3' miRNA: 3'- -AGCUUUa---GGAGCAGCG-------------CGUCGu--GCGC- -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 26247 | 0.69 | 0.459223 |
Target: 5'- aCGccGUCU---UCGCGCGGCACGUGg -3' miRNA: 3'- aGCuuUAGGagcAGCGCGUCGUGCGC- -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 21839 | 0.69 | 0.449023 |
Target: 5'- cCGgcAUCCUgcaCGCaGCAGCGCGCGu -3' miRNA: 3'- aGCuuUAGGAgcaGCG-CGUCGUGCGC- -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 9874 | 0.69 | 0.429008 |
Target: 5'- cUCGGAGUaCCUgaGcCGCGagaaGGCGCGCGa -3' miRNA: 3'- -AGCUUUA-GGAg-CaGCGCg---UCGUGCGC- -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 26386 | 0.7 | 0.403791 |
Target: 5'- cUCGAAugaCCUCGccgccaccguggagCGCGCGGguCGCGg -3' miRNA: 3'- -AGCUUua-GGAGCa-------------GCGCGUCguGCGC- -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 10535 | 0.71 | 0.354523 |
Target: 5'- gCGugguAGUCCUCGgguacaaauUUGCGCAgGUACGCGg -3' miRNA: 3'- aGCu---UUAGGAGC---------AGCGCGU-CGUGCGC- -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 7117 | 0.71 | 0.345877 |
Target: 5'- cUUGAAAUCCgaGUCGuCGCAGC-CGCc -3' miRNA: 3'- -AGCUUUAGGagCAGC-GCGUCGuGCGc -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 24549 | 0.73 | 0.267825 |
Target: 5'- uUCGAGGaCCaaUCG-CGCccGCAGCACGCGg -3' miRNA: 3'- -AGCUUUaGG--AGCaGCG--CGUCGUGCGC- -5' |
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8331 | 5' | -54.6 | NC_002067.1 | + | 24120 | 1.08 | 0.000782 |
Target: 5'- cUCGAAAUCCUCGUCGCGCAGCACGCGc -3' miRNA: 3'- -AGCUUUAGGAGCAGCGCGUCGUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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