Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8332 | 3' | -52.9 | NC_002067.1 | + | 6993 | 0.66 | 0.750639 |
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Target: 5'- gGUUCACGGccUUGUagggaCAGCAgcCCUUCUCc -3' miRNA: 3'- gCAAGUGCU--AGCG-----GUCGU--GGAAGAGc -5' |
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| 8332 | 3' | -52.9 | NC_002067.1 | + | 8476 | 0.66 | 0.706477 |
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Target: 5'- gCGUcgUCGCGcaCGCCAGCGC--UCUCa -3' miRNA: 3'- -GCA--AGUGCuaGCGGUCGUGgaAGAGc -5' |
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| 8332 | 3' | -52.9 | NC_002067.1 | + | 8767 | 0.67 | 0.695206 |
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Target: 5'- gCGUcuggaACGAgagCgGCCugGGCGCCUUCUCGc -3' miRNA: 3'- -GCAag---UGCUa--G-CGG--UCGUGGAAGAGC- -5' |
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| 8332 | 3' | -52.9 | NC_002067.1 | + | 9366 | 0.67 | 0.661046 |
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Target: 5'- ----gAUGcAUCGUCAGCGCCcUCUCGc -3' miRNA: 3'- gcaagUGC-UAGCGGUCGUGGaAGAGC- -5' |
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| 8332 | 3' | -52.9 | NC_002067.1 | + | 5855 | 0.69 | 0.562697 |
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Target: 5'- uGUcCACGAgCGCCAGCugUUgggguagguauucccUCUCGa -3' miRNA: 3'- gCAaGUGCUaGCGGUCGugGA---------------AGAGC- -5' |
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| 8332 | 3' | -52.9 | NC_002067.1 | + | 11586 | 0.69 | 0.523605 |
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Target: 5'- --cUCGCGGUCGCuCAGCucgcgccGCCggCUCGc -3' miRNA: 3'- gcaAGUGCUAGCG-GUCG-------UGGaaGAGC- -5' |
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| 8332 | 3' | -52.9 | NC_002067.1 | + | 11380 | 0.7 | 0.502819 |
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Target: 5'- aGUaguUGAUUGCCGcCACCUUCUCGg -3' miRNA: 3'- gCAaguGCUAGCGGUcGUGGAAGAGC- -5' |
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| 8332 | 3' | -52.9 | NC_002067.1 | + | 12021 | 1.09 | 0.00109 |
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Target: 5'- gCGUUCACGAUCGCCAGCACCUUCUCGu -3' miRNA: 3'- -GCAAGUGCUAGCGGUCGUGGAAGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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