Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8333 | 3' | -62.4 | NC_002067.1 | + | 8357 | 0.66 | 0.265231 |
Target: 5'- gGGCAGGgGCGGCagaggcacguCGGCguggCGCUCggGCa -3' miRNA: 3'- -UCGUUCgCGCCG----------GCCGa---GUGGGa-CGg -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 11637 | 0.66 | 0.265231 |
Target: 5'- uGGC-AGC-CGaGCCGGCgCGCCUuggagGCCg -3' miRNA: 3'- -UCGuUCGcGC-CGGCCGaGUGGGa----CGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 22814 | 0.66 | 0.258568 |
Target: 5'- cGC-AGCGCGcugauaaaGuuGGUguaaUCAUCCUGCCc -3' miRNA: 3'- uCGuUCGCGC--------CggCCG----AGUGGGACGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 15356 | 0.66 | 0.258568 |
Target: 5'- cGCAAGagccgGCGGgCGGacggaUCGCCCaggcGCCa -3' miRNA: 3'- uCGUUCg----CGCCgGCCg----AGUGGGa---CGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 28757 | 0.66 | 0.252043 |
Target: 5'- uGCucGCGCaaGGgCGGCagACUCUGCUg -3' miRNA: 3'- uCGuuCGCG--CCgGCCGagUGGGACGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 19778 | 0.66 | 0.245654 |
Target: 5'- gAGUAuaguuGCGCGcGUCGGCgacCACCgaGUCg -3' miRNA: 3'- -UCGUu----CGCGC-CGGCCGa--GUGGgaCGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 16098 | 0.66 | 0.245654 |
Target: 5'- cAGCAGGUGCuGCCccaGGCggUGCUgCUGCCg -3' miRNA: 3'- -UCGUUCGCGcCGG---CCGa-GUGG-GACGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 2277 | 0.66 | 0.23328 |
Target: 5'- uGC-AGUGUcgGGCgGGaggCACCCUGCUg -3' miRNA: 3'- uCGuUCGCG--CCGgCCga-GUGGGACGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 18515 | 0.66 | 0.23328 |
Target: 5'- gGGCGccUGCGGCUGGCguagUACCgggUGCCg -3' miRNA: 3'- -UCGUucGCGCCGGCCGa---GUGGg--ACGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 22546 | 0.66 | 0.227292 |
Target: 5'- uAGCAGGUGacccaGGUacgaguugCGGUUUACgCUGCCa -3' miRNA: 3'- -UCGUUCGCg----CCG--------GCCGAGUGgGACGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 2078 | 0.67 | 0.221434 |
Target: 5'- -cCAuGUGCuGCUGGCuUUACCCUGCUc -3' miRNA: 3'- ucGUuCGCGcCGGCCG-AGUGGGACGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 5186 | 0.67 | 0.209552 |
Target: 5'- uGCuGGUGCugaaacgGGCaCGGUcuUCGCCCUGCg -3' miRNA: 3'- uCGuUCGCG-------CCG-GCCG--AGUGGGACGg -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 10516 | 0.67 | 0.20463 |
Target: 5'- cGGCGAGCG-GGaCUuGgUCACCCcGCCg -3' miRNA: 3'- -UCGUUCGCgCC-GGcCgAGUGGGaCGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 5941 | 0.67 | 0.20463 |
Target: 5'- gAGgAGGauuuGauGuuGGCUUGCCCUGCCg -3' miRNA: 3'- -UCgUUCg---CgcCggCCGAGUGGGACGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 11513 | 0.67 | 0.199278 |
Target: 5'- cGUGAGCGCGaGCCGGCg-GCgCgaGCUg -3' miRNA: 3'- uCGUUCGCGC-CGGCCGagUG-GgaCGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 15197 | 0.67 | 0.194049 |
Target: 5'- -aCAAGCGCGGgCGGaCUgcCACCgCcGCCg -3' miRNA: 3'- ucGUUCGCGCCgGCC-GA--GUGG-GaCGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 5060 | 0.67 | 0.194049 |
Target: 5'- gGGCGuccAGCGCGGCCaGCgUCAugUCCUuCCa -3' miRNA: 3'- -UCGU---UCGCGCCGGcCG-AGU--GGGAcGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 17546 | 0.68 | 0.179078 |
Target: 5'- aGGuCAAGgGUGGCgGGUUucauuaCACCCgugGCCa -3' miRNA: 3'- -UC-GUUCgCGCCGgCCGA------GUGGGa--CGG- -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 17671 | 0.68 | 0.17432 |
Target: 5'- cGCAucuGGcCGCGGCCGcuuCUCGCCCUucgGCg -3' miRNA: 3'- uCGU---UC-GCGCCGGCc--GAGUGGGA---CGg -5' |
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8333 | 3' | -62.4 | NC_002067.1 | + | 13126 | 0.69 | 0.156398 |
Target: 5'- cGGCAGG-GUGaGCCGGC-CGCgCUUGCUg -3' miRNA: 3'- -UCGUUCgCGC-CGGCCGaGUG-GGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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