Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8337 | 3' | -60.3 | NC_002067.1 | + | 15825 | 0.66 | 0.32426 |
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Target: 5'- gCGCgagUUCGCUCcgCGGCGGCGc-- -3' miRNA: 3'- gGCGaa-GAGCGGGaaGCCGCCGCcuu -5' |
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| 8337 | 3' | -60.3 | NC_002067.1 | + | 16863 | 0.66 | 0.316405 |
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Target: 5'- cCUGCgcaUgGCCCgccgcCGGCGGCGGu- -3' miRNA: 3'- -GGCGaagAgCGGGaa---GCCGCCGCCuu -5' |
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| 8337 | 3' | -60.3 | NC_002067.1 | + | 17315 | 0.72 | 0.13582 |
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Target: 5'- cCCGCggC-CGCCCa-CGGUGGUGGAGa -3' miRNA: 3'- -GGCGaaGaGCGGGaaGCCGCCGCCUU- -5' |
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| 8337 | 3' | -60.3 | NC_002067.1 | + | 23921 | 0.72 | 0.128459 |
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Target: 5'- gCCGCgcCUCGCCCUUCagGGuCGaGUGGAGc -3' miRNA: 3'- -GGCGaaGAGCGGGAAG--CC-GC-CGCCUU- -5' |
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| 8337 | 3' | -60.3 | NC_002067.1 | + | 12987 | 0.72 | 0.120789 |
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Target: 5'- gUCGUa-CUCGCCCguauagaacccCGGCGGCGGAAg -3' miRNA: 3'- -GGCGaaGAGCGGGaa---------GCCGCCGCCUU- -5' |
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| 8337 | 3' | -60.3 | NC_002067.1 | + | 17416 | 1.07 | 0.000234 |
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Target: 5'- gCCGCUUCUCGCCCUUCGGCGGCGGAAg -3' miRNA: 3'- -GGCGAAGAGCGGGAAGCCGCCGCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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