Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8389 | 3' | -37.8 | NC_002072.2 | + | 16895 | 0.66 | 1 |
Target: 5'- ---cUUGGGUAGAUAauAAAGAaguAGCUUCa -3' miRNA: 3'- uaaaAAUCCGUUUAU--UUUUU---UCGGGG- -5' |
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8389 | 3' | -37.8 | NC_002072.2 | + | 22472 | 0.67 | 0.999999 |
Target: 5'- ----gUGGGUAguAAUGAGAAAAugguGUCCCa -3' miRNA: 3'- uaaaaAUCCGU--UUAUUUUUUU----CGGGG- -5' |
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8389 | 3' | -37.8 | NC_002072.2 | + | 34636 | 0.68 | 0.999995 |
Target: 5'- ----gUGGGCGGGUuu-AAuAGCCCg -3' miRNA: 3'- uaaaaAUCCGUUUAuuuUUuUCGGGg -5' |
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8389 | 3' | -37.8 | NC_002072.2 | + | 11505 | 0.7 | 0.999945 |
Target: 5'- -------uGUAAAUuaaGGAGAAAGCCCCa -3' miRNA: 3'- uaaaaaucCGUUUA---UUUUUUUCGGGG- -5' |
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8389 | 3' | -37.8 | NC_002072.2 | + | 19970 | 0.7 | 0.999945 |
Target: 5'- uUUUUUAGGCGuGUAAucAAuccacGGCuCCCa -3' miRNA: 3'- uAAAAAUCCGUuUAUUuuUU-----UCG-GGG- -5' |
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8389 | 3' | -37.8 | NC_002072.2 | + | 18403 | 0.71 | 0.999851 |
Target: 5'- ------cGGCuuGAAUAuucAGGAAGCCCCu -3' miRNA: 3'- uaaaaauCCG--UUUAUu--UUUUUCGGGG- -5' |
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8389 | 3' | -37.8 | NC_002072.2 | + | 1045 | 0.73 | 0.997876 |
Target: 5'- aAUUUUUAGGgAAAUAAAAAGAuGUCUa -3' miRNA: 3'- -UAAAAAUCCgUUUAUUUUUUU-CGGGg -5' |
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8389 | 3' | -37.8 | NC_002072.2 | + | 3297 | 0.74 | 0.996747 |
Target: 5'- ----cUGGGUcAGUAauaucAAAAAAGCCCCc -3' miRNA: 3'- uaaaaAUCCGuUUAU-----UUUUUUCGGGG- -5' |
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8389 | 3' | -37.8 | NC_002072.2 | + | 1855 | 0.8 | 0.918547 |
Target: 5'- uGUUUcUGGGCAAGUAAAGGu-GCCCa -3' miRNA: 3'- -UAAAaAUCCGUUUAUUUUUuuCGGGg -5' |
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8389 | 3' | -37.8 | NC_002072.2 | + | 33568 | 0.83 | 0.826418 |
Target: 5'- ------uGGUAAAUAAAAAAAGCCCa -3' miRNA: 3'- uaaaaauCCGUUUAUUUUUUUCGGGg -5' |
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8389 | 3' | -37.8 | NC_002072.2 | + | 22890 | 1.16 | 0.018793 |
Target: 5'- gAUUUUUAGGCAAAUAAAAAAAGCCCCa -3' miRNA: 3'- -UAAAAAUCCGUUUAUUUUUUUCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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