Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 840 | 5' | -54.5 | NC_000898.1 | + | 3031 | 0.66 | 0.957524 |
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Target: 5'- gACGAgGGCGccgGCGGgaaCGCCACGGg- -3' miRNA: 3'- -UGCUaUUGU---UGCCaggGCGGUGCCac -5' |
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| 840 | 5' | -54.5 | NC_000898.1 | + | 156198 | 0.66 | 0.957524 |
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Target: 5'- gACGAgGGCGccgGCGGgaaCGCCACGGg- -3' miRNA: 3'- -UGCUaUUGU---UGCCaggGCGGUGCCac -5' |
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| 840 | 5' | -54.5 | NC_000898.1 | + | 4364 | 0.66 | 0.949608 |
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Target: 5'- -gGGUGGCGcgucGCGaaGUCCCGCCucauaGGUGg -3' miRNA: 3'- ugCUAUUGU----UGC--CAGGGCGGug---CCAC- -5' |
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| 840 | 5' | -54.5 | NC_000898.1 | + | 157531 | 0.66 | 0.949608 |
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Target: 5'- -gGGUGGCGcgucGCGaaGUCCCGCCucauaGGUGg -3' miRNA: 3'- ugCUAUUGU----UGC--CAGGGCGGug---CCAC- -5' |
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| 840 | 5' | -54.5 | NC_000898.1 | + | 7162 | 1.08 | 0.004971 |
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Target: 5'- aACGAUAACAACGGUCCCGCCACGGUGa -3' miRNA: 3'- -UGCUAUUGUUGCCAGGGCGGUGCCAC- -5' |
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| 840 | 5' | -54.5 | NC_000898.1 | + | 160329 | 1.08 | 0.004971 |
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Target: 5'- aACGAUAACAACGGUCCCGCCACGGUGa -3' miRNA: 3'- -UGCUAUUGUUGCCAGGGCGGUGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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