miRNA: 3'- -GGGAGAuuuuuGGGACUCuUAGUUcg -5'
miRNA: 3'- gGGaGAUU--------UUUGGGACUCUUaGUUCG- -5'
miRNA: 3'- -GGGAGAUUUUUGGGACUCUUAGUUCG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8404 | 3' | -44.7 | NC_002160.2 | + | 3010 | 0.67 | 0.637436 |
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Target: 5'- gCCCUCUucu--UCCUGAGcGUCGAc- -3' miRNA: 3'- -GGGAGAuuuuuGGGACUCuUAGUUcg -5' |
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| 8404 | 3' | -44.7 | NC_002160.2 | + | 4815 | 0.71 | 0.387382 |
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Target: 5'- aCCaCUAAcacaagccGAAUCCUGGGAAaCAGGCa -3' miRNA: 3'- gGGaGAUU--------UUUGGGACUCUUaGUUCG- -5' |
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| 8404 | 3' | -44.7 | NC_002160.2 | + | 1170 | 1.18 | 0.000157 |
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Target: 5'- cCCCUCUAAAAACCCUGAGAAUCAAGCa -3' miRNA: 3'- -GGGAGAUUUUUGGGACUCUUAGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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