Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 841 | 3' | -56.9 | NC_000898.1 | + | 5547 | 0.66 | 0.902422 |
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Target: 5'- uCCCuccccGCCgCUCGCCCGUgugcgcauagcgUCgCCGGu -3' miRNA: 3'- uGGGuaua-UGG-GAGCGGGCA------------AG-GGCC- -5' |
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| 841 | 3' | -56.9 | NC_000898.1 | + | 158715 | 0.66 | 0.902422 |
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Target: 5'- uCCCuccccGCCgCUCGCCCGUgugcgcauagcgUCgCCGGu -3' miRNA: 3'- uGGGuaua-UGG-GAGCGGGCA------------AG-GGCC- -5' |
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| 841 | 3' | -56.9 | NC_000898.1 | + | 161468 | 0.67 | 0.868784 |
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Target: 5'- cCCCGUAUGCuCCcCGCCUGcccaCCCGc -3' miRNA: 3'- uGGGUAUAUG-GGaGCGGGCaa--GGGCc -5' |
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| 841 | 3' | -56.9 | NC_000898.1 | + | 155464 | 0.67 | 0.868784 |
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Target: 5'- cGCCCGUGgugGCCgUCuGUCUGUUCCUc- -3' miRNA: 3'- -UGGGUAUa--UGGgAG-CGGGCAAGGGcc -5' |
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| 841 | 3' | -56.9 | NC_000898.1 | + | 8301 | 0.67 | 0.868784 |
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Target: 5'- cCCCGUAUGCuCCcCGCCUGcccaCCCGc -3' miRNA: 3'- uGGGUAUAUG-GGaGCGGGCaa--GGGCc -5' |
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| 841 | 3' | -56.9 | NC_000898.1 | + | 2297 | 0.67 | 0.868784 |
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Target: 5'- cGCCCGUGgugGCCgUCuGUCUGUUCCUc- -3' miRNA: 3'- -UGGGUAUa--UGGgAG-CGGGCAAGGGcc -5' |
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| 841 | 3' | -56.9 | NC_000898.1 | + | 3001 | 0.67 | 0.846125 |
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Target: 5'- uGCCCGgaggacACCCacggcuUUGCUCGgagUCCCGGa -3' miRNA: 3'- -UGGGUaua---UGGG------AGCGGGCa--AGGGCC- -5' |
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| 841 | 3' | -56.9 | NC_000898.1 | + | 156168 | 0.67 | 0.846125 |
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Target: 5'- uGCCCGgaggacACCCacggcuUUGCUCGgagUCCCGGa -3' miRNA: 3'- -UGGGUaua---UGGG------AGCGGGCa--AGGGCC- -5' |
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| 841 | 3' | -56.9 | NC_000898.1 | + | 9217 | 0.7 | 0.701995 |
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Target: 5'- aGCUCAUG-GCCCUCGCggCCGUUCUCu- -3' miRNA: 3'- -UGGGUAUaUGGGAGCG--GGCAAGGGcc -5' |
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| 841 | 3' | -56.9 | NC_000898.1 | + | 9050 | 1.11 | 0.002306 |
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Target: 5'- cACCCAUAUACCCUCGCCCGUUCCCGGg -3' miRNA: 3'- -UGGGUAUAUGGGAGCGGGCAAGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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