Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8417 | 5' | -54.4 | NC_002166.1 | + | 208 | 0.67 | 0.600662 |
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Target: 5'- cUGGACGCCUGCCGACcuucuAGCCGc---- -3' miRNA: 3'- -AUUUGCGGGCGGCUGu----UUGGUccuga -5' |
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| 8417 | 5' | -54.4 | NC_002166.1 | + | 32266 | 0.67 | 0.5667 |
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Target: 5'- gAAAUGCgCCGCCGugAAcGCgAGGAa- -3' miRNA: 3'- aUUUGCG-GGCGGCugUU-UGgUCCUga -5' |
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| 8417 | 5' | -54.4 | NC_002166.1 | + | 10639 | 0.68 | 0.522251 |
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Target: 5'- ---cCGCgCCGCUGACGGAUCGcuGGGCg -3' miRNA: 3'- auuuGCG-GGCGGCUGUUUGGU--CCUGa -5' |
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| 8417 | 5' | -54.4 | NC_002166.1 | + | 1750 | 0.69 | 0.458355 |
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Target: 5'- cUGAGCcuCCCGCUGGuCGAguucgGCCAGGGCUu -3' miRNA: 3'- -AUUUGc-GGGCGGCU-GUU-----UGGUCCUGA- -5' |
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| 8417 | 5' | -54.4 | NC_002166.1 | + | 11109 | 0.72 | 0.303529 |
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Target: 5'- -uGGCGCCCGCCGcuuGAGCCucucGGGCUu -3' miRNA: 3'- auUUGCGGGCGGCug-UUUGGu---CCUGA- -5' |
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| 8417 | 5' | -54.4 | NC_002166.1 | + | 2181 | 1.06 | 0.001124 |
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Target: 5'- cUAAACGCCCGCCGACAAACCAGGACUg -3' miRNA: 3'- -AUUUGCGGGCGGCUGUUUGGUCCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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