Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8419 | 3' | -46.1 | NC_002166.1 | + | 470 | 0.67 | 0.984711 |
Target: 5'- aCUuUCGCCGUugGUUGcguggGAGUGGaucuGGa -3' miRNA: 3'- -GAuAGCGGUAugCAAU-----UUUACCg---CCc -5' |
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8419 | 3' | -46.1 | NC_002166.1 | + | 15275 | 0.68 | 0.971417 |
Target: 5'- ---gCGCCA-ACGUUGu--UGGCGGu -3' miRNA: 3'- gauaGCGGUaUGCAAUuuuACCGCCc -5' |
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8419 | 3' | -46.1 | NC_002166.1 | + | 23260 | 0.68 | 0.971417 |
Target: 5'- -gAUUGCCAUGCGUaAGGcUGGCa-- -3' miRNA: 3'- gaUAGCGGUAUGCAaUUUuACCGccc -5' |
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8419 | 3' | -46.1 | NC_002166.1 | + | 7552 | 0.68 | 0.967984 |
Target: 5'- -cAUCGCCAacUGCGgcugAAAAUGGUGc- -3' miRNA: 3'- gaUAGCGGU--AUGCaa--UUUUACCGCcc -5' |
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8419 | 3' | -46.1 | NC_002166.1 | + | 13490 | 0.69 | 0.935604 |
Target: 5'- -cAUCG-UAUGCGUU----UGGCGGGg -3' miRNA: 3'- gaUAGCgGUAUGCAAuuuuACCGCCC- -5' |
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8419 | 3' | -46.1 | NC_002166.1 | + | 33227 | 0.7 | 0.929716 |
Target: 5'- cCUGUCGCUGUA-GccAAAAUGGCGuGGc -3' miRNA: 3'- -GAUAGCGGUAUgCaaUUUUACCGC-CC- -5' |
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8419 | 3' | -46.1 | NC_002166.1 | + | 11079 | 0.71 | 0.879495 |
Target: 5'- -gAUCGCCAUGCGggcgUGAcggcguuucAGUGGCGc- -3' miRNA: 3'- gaUAGCGGUAUGCa---AUU---------UUACCGCcc -5' |
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8419 | 3' | -46.1 | NC_002166.1 | + | 8646 | 0.71 | 0.879495 |
Target: 5'- ----aGCCAU-CGgagccGGAGUGGCGGGg -3' miRNA: 3'- gauagCGGUAuGCaa---UUUUACCGCCC- -5' |
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8419 | 3' | -46.1 | NC_002166.1 | + | 3290 | 0.75 | 0.70459 |
Target: 5'- -cAUCGCCAccCGgc--AAUGGCGGGa -3' miRNA: 3'- gaUAGCGGUauGCaauuUUACCGCCC- -5' |
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8419 | 3' | -46.1 | NC_002166.1 | + | 17257 | 0.83 | 0.283471 |
Target: 5'- gCUAUCGCCugaGCGUUAGugguGAUGGCGGa -3' miRNA: 3'- -GAUAGCGGua-UGCAAUU----UUACCGCCc -5' |
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8419 | 3' | -46.1 | NC_002166.1 | + | 5264 | 1.12 | 0.004755 |
Target: 5'- uCUAUCGCCAUACGUUAAAAUGGCGGGu -3' miRNA: 3'- -GAUAGCGGUAUGCAAUUUUACCGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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