Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8422 | 5' | -47.6 | NC_002166.1 | + | 35653 | 0.66 | 0.963756 |
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Target: 5'- --uUCAGCAGuAUcGCCUGCgUCCUGa -3' miRNA: 3'- gcuAGUUGUUuUGaUGGAUGgAGGGC- -5' |
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| 8422 | 5' | -47.6 | NC_002166.1 | + | 1161 | 0.68 | 0.929327 |
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Target: 5'- aGGUCAGCAucguuuGCUGCCUGCgUaCUGa -3' miRNA: 3'- gCUAGUUGUuu----UGAUGGAUGgAgGGC- -5' |
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| 8422 | 5' | -47.6 | NC_002166.1 | + | 2472 | 0.68 | 0.929327 |
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Target: 5'- aGA--AACAGAGCUGCaucgucaugGCCUCCCu -3' miRNA: 3'- gCUagUUGUUUUGAUGga-------UGGAGGGc -5' |
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| 8422 | 5' | -47.6 | NC_002166.1 | + | 20959 | 0.69 | 0.895304 |
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Target: 5'- gCGAUUAuaguuuCAcuGCUGCUgGCCUCCCu -3' miRNA: 3'- -GCUAGUu-----GUuuUGAUGGaUGGAGGGc -5' |
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| 8422 | 5' | -47.6 | NC_002166.1 | + | 11548 | 0.69 | 0.871196 |
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Target: 5'- aGGUUAGCAAccgaaaguuucGGCUggGCaCUACUUCCCGa -3' miRNA: 3'- gCUAGUUGUU-----------UUGA--UG-GAUGGAGGGC- -5' |
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| 8422 | 5' | -47.6 | NC_002166.1 | + | 10852 | 1.1 | 0.004299 |
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Target: 5'- cCGAUCAACAAAACUACCUACCUCCCGc -3' miRNA: 3'- -GCUAGUUGUUUUGAUGGAUGGAGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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