Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8423 | 3' | -54.5 | NC_002166.1 | + | 6542 | 0.66 | 0.688105 |
Target: 5'- --uUCCGUguCgGggUaaUGgGCGGUGCc -3' miRNA: 3'- uauAGGCAguGgCuuG--ACgCGCCACG- -5' |
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8423 | 3' | -54.5 | NC_002166.1 | + | 17212 | 0.66 | 0.670122 |
Target: 5'- ---aCCGUCGCCGuuaugccguuuauCUGCGUGGccguagccUGCu -3' miRNA: 3'- uauaGGCAGUGGCuu-----------GACGCGCC--------ACG- -5' |
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8423 | 3' | -54.5 | NC_002166.1 | + | 17432 | 0.66 | 0.64298 |
Target: 5'- ---gCUGUUACCG-GCUGCcGUGGUGa -3' miRNA: 3'- uauaGGCAGUGGCuUGACG-CGCCACg -5' |
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8423 | 3' | -54.5 | NC_002166.1 | + | 36998 | 0.68 | 0.541814 |
Target: 5'- uUAUCCG-CugUGGcuACgGUGCGGUGUg -3' miRNA: 3'- uAUAGGCaGugGCU--UGaCGCGCCACG- -5' |
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8423 | 3' | -54.5 | NC_002166.1 | + | 11782 | 0.69 | 0.456615 |
Target: 5'- -cAUCCGgaacaagUACCGAACcgGCGaCGGcUGCg -3' miRNA: 3'- uaUAGGCa------GUGGCUUGa-CGC-GCC-ACG- -5' |
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8423 | 3' | -54.5 | NC_002166.1 | + | 11020 | 1.11 | 0.000594 |
Target: 5'- gAUAUCCGUCACCGAACUGCGCGGUGCg -3' miRNA: 3'- -UAUAGGCAGUGGCUUGACGCGCCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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