Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8424 | 5' | -53.8 | NC_002166.1 | + | 8134 | 0.66 | 0.691629 |
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Target: 5'- aAGCAGugCuaCCGUCAggCCGCCAucCAGu -3' miRNA: 3'- -UCGUUugG--GGCAGUuaGGUGGUc-GUC- -5' |
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| 8424 | 5' | -53.8 | NC_002166.1 | + | 10531 | 0.66 | 0.691629 |
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Target: 5'- aGGCucGCCCCGUaggacugaaucgCAGUaCCGCUgcuGGCGGa -3' miRNA: 3'- -UCGuuUGGGGCA------------GUUA-GGUGG---UCGUC- -5' |
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| 8424 | 5' | -53.8 | NC_002166.1 | + | 33868 | 0.67 | 0.646022 |
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Target: 5'- --gAAGCCCC----AUCCAUCAGCAGa -3' miRNA: 3'- ucgUUUGGGGcaguUAGGUGGUCGUC- -5' |
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| 8424 | 5' | -53.8 | NC_002166.1 | + | 1778 | 0.67 | 0.611628 |
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Target: 5'- cGGCAuccuUCCUgaacugGUCAAUgCGCCAGCGGu -3' miRNA: 3'- -UCGUuu--GGGG------CAGUUAgGUGGUCGUC- -5' |
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| 8424 | 5' | -53.8 | NC_002166.1 | + | 10708 | 0.68 | 0.532499 |
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Target: 5'- cGCAcGCCCag-CGAUCCGUCAGCGGc -3' miRNA: 3'- uCGUuUGGGgcaGUUAGGUGGUCGUC- -5' |
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| 8424 | 5' | -53.8 | NC_002166.1 | + | 39084 | 0.69 | 0.51051 |
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Target: 5'- gGGCu-GCCCCGUC-AUCgACCAGg-- -3' miRNA: 3'- -UCGuuUGGGGCAGuUAGgUGGUCguc -5' |
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| 8424 | 5' | -53.8 | NC_002166.1 | + | 4739 | 0.7 | 0.447064 |
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Target: 5'- uGGCAAccgccuaugacACCUCGUUGAaugCCACCGGCGa -3' miRNA: 3'- -UCGUU-----------UGGGGCAGUUa--GGUGGUCGUc -5' |
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| 8424 | 5' | -53.8 | NC_002166.1 | + | 10429 | 0.7 | 0.436915 |
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Target: 5'- cGGUGGugCCgcauCGUCu-UCCGCCAGCAGc -3' miRNA: 3'- -UCGUUugGG----GCAGuuAGGUGGUCGUC- -5' |
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| 8424 | 5' | -53.8 | NC_002166.1 | + | 13433 | 1.08 | 0.000993 |
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Target: 5'- cAGCAAACCCCGUCAAUCCACCAGCAGc -3' miRNA: 3'- -UCGUUUGGGGCAGUUAGGUGGUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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