Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8425 | 5' | -48.3 | NC_002166.1 | + | 18167 | 0.66 | 0.956663 |
Target: 5'- cCCAGCCCacgccaguaAUAUCuuAcCGGAACGGCg -3' miRNA: 3'- aGGUCGGGc--------UGUAGuuU-GCUUUGUUG- -5' |
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8425 | 5' | -48.3 | NC_002166.1 | + | 19056 | 0.66 | 0.936652 |
Target: 5'- aCCGGCaCgGGCA-CGAACuGGACGGCa -3' miRNA: 3'- aGGUCG-GgCUGUaGUUUGcUUUGUUG- -5' |
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8425 | 5' | -48.3 | NC_002166.1 | + | 6849 | 0.67 | 0.91831 |
Target: 5'- gCCAGCCUGAUAgc--GCGAucAAUAGCc -3' miRNA: 3'- aGGUCGGGCUGUaguuUGCU--UUGUUG- -5' |
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8425 | 5' | -48.3 | NC_002166.1 | + | 17426 | 0.67 | 0.904484 |
Target: 5'- aCCGGCugCCGugGUgAcGGCGAAGCGAUc -3' miRNA: 3'- aGGUCG--GGCugUAgU-UUGCUUUGUUG- -5' |
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8425 | 5' | -48.3 | NC_002166.1 | + | 19304 | 0.68 | 0.881403 |
Target: 5'- gCCAGCCC---AUCAAcCGAGAUAAUg -3' miRNA: 3'- aGGUCGGGcugUAGUUuGCUUUGUUG- -5' |
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8425 | 5' | -48.3 | NC_002166.1 | + | 38116 | 0.68 | 0.873107 |
Target: 5'- -aCAGCCCauGACAggCAGACGAugauGCAGa -3' miRNA: 3'- agGUCGGG--CUGUa-GUUUGCUu---UGUUg -5' |
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8425 | 5' | -48.3 | NC_002166.1 | + | 24791 | 0.68 | 0.864521 |
Target: 5'- aUCCAGCaccuCCGAUA-CGGAauugcCGGAACAGCg -3' miRNA: 3'- -AGGUCG----GGCUGUaGUUU-----GCUUUGUUG- -5' |
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8425 | 5' | -48.3 | NC_002166.1 | + | 8351 | 0.7 | 0.807463 |
Target: 5'- aCCAGCCCGGCAccccacUCAAcCc--GCAACa -3' miRNA: 3'- aGGUCGGGCUGU------AGUUuGcuuUGUUG- -5' |
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8425 | 5' | -48.3 | NC_002166.1 | + | 7223 | 0.71 | 0.742774 |
Target: 5'- aUCCAGUCa-ACAUCGAAUGAguAGCGAUa -3' miRNA: 3'- -AGGUCGGgcUGUAGUUUGCU--UUGUUG- -5' |
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8425 | 5' | -48.3 | NC_002166.1 | + | 14267 | 0.73 | 0.637933 |
Target: 5'- aCCGGCaaaGAUAUCuaccuGGACGGAACAGCg -3' miRNA: 3'- aGGUCGgg-CUGUAG-----UUUGCUUUGUUG- -5' |
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8425 | 5' | -48.3 | NC_002166.1 | + | 15589 | 1.12 | 0.002153 |
Target: 5'- gUCCAGCCCGACAUCAAACGAAACAACg -3' miRNA: 3'- -AGGUCGGGCUGUAGUUUGCUUUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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