Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8426 | 5' | -55.3 | NC_002166.1 | + | 34495 | 0.66 | 0.664476 |
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Target: 5'- uCGAgCGggcuUUAACGuGCGCUaacUGCGGUCa -3' miRNA: 3'- -GCUgGC----AAUUGC-CGCGAcu-ACGCCGG- -5' |
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| 8426 | 5' | -55.3 | NC_002166.1 | + | 10639 | 0.66 | 0.642211 |
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Target: 5'- cCGcGCCGcUGACGGauCGCUGGgcgUGCGuGCUa -3' miRNA: 3'- -GC-UGGCaAUUGCC--GCGACU---ACGC-CGG- -5' |
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| 8426 | 5' | -55.3 | NC_002166.1 | + | 2234 | 0.67 | 0.608767 |
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Target: 5'- uCGACUGUg--UGGCGCUGc-GUGaGCCu -3' miRNA: 3'- -GCUGGCAauuGCCGCGACuaCGC-CGG- -5' |
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| 8426 | 5' | -55.3 | NC_002166.1 | + | 34896 | 0.67 | 0.586558 |
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Target: 5'- uGACCGUUGGCGaGaGCuUGAagaagcUGCGGUUa -3' miRNA: 3'- gCUGGCAAUUGC-CgCG-ACU------ACGCCGG- -5' |
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| 8426 | 5' | -55.3 | NC_002166.1 | + | 786 | 0.67 | 0.553559 |
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Target: 5'- aGAUUGUcAGCGGUGCacucagccggGAacagGCGGCCa -3' miRNA: 3'- gCUGGCAaUUGCCGCGa---------CUa---CGCCGG- -5' |
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| 8426 | 5' | -55.3 | NC_002166.1 | + | 1460 | 0.69 | 0.458744 |
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Target: 5'- aCGACU--UAACGGCGCUGGUuaucGCuGGUg -3' miRNA: 3'- -GCUGGcaAUUGCCGCGACUA----CG-CCGg -5' |
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| 8426 | 5' | -55.3 | NC_002166.1 | + | 10698 | 0.72 | 0.314712 |
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Target: 5'- gCGAuCCGUcAGCGGCGCgGGUcaGUGGCa -3' miRNA: 3'- -GCU-GGCAaUUGCCGCGaCUA--CGCCGg -5' |
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| 8426 | 5' | -55.3 | NC_002166.1 | + | 31119 | 0.73 | 0.291822 |
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Target: 5'- -aAUCGUUAACGGCGuCUGuugGCGGUUu -3' miRNA: 3'- gcUGGCAAUUGCCGC-GACua-CGCCGG- -5' |
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| 8426 | 5' | -55.3 | NC_002166.1 | + | 19177 | 0.77 | 0.145594 |
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Target: 5'- uGACgGUUAcuccagcugcuucccCGGCGCUGA-GCGGCCa -3' miRNA: 3'- gCUGgCAAUu--------------GCCGCGACUaCGCCGG- -5' |
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| 8426 | 5' | -55.3 | NC_002166.1 | + | 15685 | 1.13 | 0.000412 |
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Target: 5'- gCGACCGUUAACGGCGCUGAUGCGGCCg -3' miRNA: 3'- -GCUGGCAAUUGCCGCGACUACGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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