Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 843 | 3' | -50 | NC_000898.1 | + | 3111 | 0.66 | 0.997841 |
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Target: 5'- gCCgcGA-CCUGCCgAUGCAcGGCGcggacggagauGCCg -3' miRNA: 3'- -GGauCUaGGACGGaUAUGU-CCGU-----------UGG- -5' |
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| 843 | 3' | -50 | NC_000898.1 | + | 156278 | 0.66 | 0.997841 |
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Target: 5'- gCCgcGA-CCUGCCgAUGCAcGGCGcggacggagauGCCg -3' miRNA: 3'- -GGauCUaGGACGGaUAUGU-CCGU-----------UGG- -5' |
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| 843 | 3' | -50 | NC_000898.1 | + | 18552 | 0.67 | 0.99337 |
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Target: 5'- -aUGGAggucUCCUGCUUaaaGUACGGGguGCa -3' miRNA: 3'- ggAUCU----AGGACGGA---UAUGUCCguUGg -5' |
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| 843 | 3' | -50 | NC_000898.1 | + | 73075 | 0.67 | 0.99337 |
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Target: 5'- aCUUGGAUCUUccGCUgcaagAUACAG-CAACCa -3' miRNA: 3'- -GGAUCUAGGA--CGGa----UAUGUCcGUUGG- -5' |
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| 843 | 3' | -50 | NC_000898.1 | + | 124235 | 0.68 | 0.991218 |
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Target: 5'- aCUUGGAgaaUCCUGCU---ACAGGCAucACa -3' miRNA: 3'- -GGAUCU---AGGACGGauaUGUCCGU--UGg -5' |
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| 843 | 3' | -50 | NC_000898.1 | + | 99273 | 1.17 | 0.004039 |
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Target: 5'- aCCUAGAUCCUGCCUAUACAGGCAACCg -3' miRNA: 3'- -GGAUCUAGGACGGAUAUGUCCGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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