miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
843 3' -50 NC_000898.1 + 3111 0.66 0.997841
Target:  5'- gCCgcGA-CCUGCCgAUGCAcGGCGcggacggagauGCCg -3'
miRNA:   3'- -GGauCUaGGACGGaUAUGU-CCGU-----------UGG- -5'
843 3' -50 NC_000898.1 + 156278 0.66 0.997841
Target:  5'- gCCgcGA-CCUGCCgAUGCAcGGCGcggacggagauGCCg -3'
miRNA:   3'- -GGauCUaGGACGGaUAUGU-CCGU-----------UGG- -5'
843 3' -50 NC_000898.1 + 18552 0.67 0.99337
Target:  5'- -aUGGAggucUCCUGCUUaaaGUACGGGguGCa -3'
miRNA:   3'- ggAUCU----AGGACGGA---UAUGUCCguUGg -5'
843 3' -50 NC_000898.1 + 73075 0.67 0.99337
Target:  5'- aCUUGGAUCUUccGCUgcaagAUACAG-CAACCa -3'
miRNA:   3'- -GGAUCUAGGA--CGGa----UAUGUCcGUUGG- -5'
843 3' -50 NC_000898.1 + 124235 0.68 0.991218
Target:  5'- aCUUGGAgaaUCCUGCU---ACAGGCAucACa -3'
miRNA:   3'- -GGAUCU---AGGACGGauaUGUCCGU--UGg -5'
843 3' -50 NC_000898.1 + 99273 1.17 0.004039
Target:  5'- aCCUAGAUCCUGCCUAUACAGGCAACCg -3'
miRNA:   3'- -GGAUCUAGGACGGAUAUGUCCGUUGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.