Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8430 | 5' | -58.2 | NC_002166.1 | + | 36272 | 0.67 | 0.432254 |
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Target: 5'- aUGUga-UGGCUGCUaUGGgGAUGGCGc -3' miRNA: 3'- -ACAaugGCCGACGGcACCaCUGCCGC- -5' |
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| 8430 | 5' | -58.2 | NC_002166.1 | + | 17262 | 0.67 | 0.432254 |
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Target: 5'- ---cGuuGGCUaucGCCugagcguuaGUGGUGAUGGCGg -3' miRNA: 3'- acaaUggCCGA---CGG---------CACCACUGCCGC- -5' |
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| 8430 | 5' | -58.2 | NC_002166.1 | + | 15820 | 0.67 | 0.394798 |
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Target: 5'- ---cACUGuaUGCCGUGGUGAC-GUGa -3' miRNA: 3'- acaaUGGCcgACGGCACCACUGcCGC- -5' |
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| 8430 | 5' | -58.2 | NC_002166.1 | + | 9728 | 0.68 | 0.359506 |
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Target: 5'- ---cACCGGCaaaaaaacUGCUGUGGUGAcCGaGCa -3' miRNA: 3'- acaaUGGCCG--------ACGGCACCACU-GC-CGc -5' |
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| 8430 | 5' | -58.2 | NC_002166.1 | + | 38349 | 0.69 | 0.326478 |
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Target: 5'- ---cGCUGGCUccgGUCGUugacuuugcacaGGUGGCGGCGa -3' miRNA: 3'- acaaUGGCCGA---CGGCA------------CCACUGCCGC- -5' |
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| 8430 | 5' | -58.2 | NC_002166.1 | + | 17431 | 1.09 | 0.000369 |
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Target: 5'- cUGUUACCGGCUGCCGUGGUGACGGCGa -3' miRNA: 3'- -ACAAUGGCCGACGGCACCACUGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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