Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8432 | 3' | -56.7 | NC_002166.1 | + | 35368 | 0.66 | 0.524273 |
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Target: 5'- gCGCGGCAGAGAauGCCAgGUaCGuauuuacGGCg- -3' miRNA: 3'- -GCGCCGUCUCU--UGGUgCGaGU-------UCGgu -5' |
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| 8432 | 3' | -56.7 | NC_002166.1 | + | 36523 | 0.67 | 0.493382 |
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Target: 5'- aGauGCAGAGAuuGCCACGgUacuGGCCGg -3' miRNA: 3'- gCgcCGUCUCU--UGGUGCgAgu-UCGGU- -5' |
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| 8432 | 3' | -56.7 | NC_002166.1 | + | 13936 | 0.67 | 0.482923 |
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Target: 5'- uGUGGCAaauGAGGuuACCGCuCUUAAGCUAg -3' miRNA: 3'- gCGCCGU---CUCU--UGGUGcGAGUUCGGU- -5' |
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| 8432 | 3' | -56.7 | NC_002166.1 | + | 10734 | 0.67 | 0.462334 |
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Target: 5'- -aUGGCAauGGGAAuacCCAgGCUCAGGCUAg -3' miRNA: 3'- gcGCCGU--CUCUU---GGUgCGAGUUCGGU- -5' |
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| 8432 | 3' | -56.7 | NC_002166.1 | + | 12299 | 0.67 | 0.452213 |
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Target: 5'- cCGC-GCGGAGAacuGCCACuGCUgGAGCg- -3' miRNA: 3'- -GCGcCGUCUCU---UGGUG-CGAgUUCGgu -5' |
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| 8432 | 3' | -56.7 | NC_002166.1 | + | 4509 | 0.69 | 0.341201 |
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Target: 5'- gGUGGCAGAGAAa-GCaCUgAAGCCAg -3' miRNA: 3'- gCGCCGUCUCUUggUGcGAgUUCGGU- -5' |
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| 8432 | 3' | -56.7 | NC_002166.1 | + | 16031 | 0.73 | 0.214134 |
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Target: 5'- gGCGGCgacugguugcccGGcGGGAUCACGCUCAccGGCCGu -3' miRNA: 3'- gCGCCG------------UC-UCUUGGUGCGAGU--UCGGU- -5' |
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| 8432 | 3' | -56.7 | NC_002166.1 | + | 5666 | 0.74 | 0.167319 |
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Target: 5'- aGCGGCAGAGAGCaGCuuUCAGGCg- -3' miRNA: 3'- gCGCCGUCUCUUGgUGcgAGUUCGgu -5' |
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| 8432 | 3' | -56.7 | NC_002166.1 | + | 29339 | 1.09 | 0.000463 |
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Target: 5'- aCGCGGCAGAGAACCACGCUCAAGCCAc -3' miRNA: 3'- -GCGCCGUCUCUUGGUGCGAGUUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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