Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8432 | 5' | -47.1 | NC_002166.1 | + | 20853 | 0.66 | 0.971943 |
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Target: 5'- aUUGGCGaUAAGUuuGCucAACGUCuucucgGGCa -3' miRNA: 3'- aAACCGC-AUUCAggUGu-UUGUAG------UCG- -5' |
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| 8432 | 5' | -47.1 | NC_002166.1 | + | 2864 | 0.66 | 0.962386 |
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Target: 5'- aUUGGCGUAAcUCCGCAGgaugccgaaaugaugGCGUCc-- -3' miRNA: 3'- aAACCGCAUUcAGGUGUU---------------UGUAGucg -5' |
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| 8432 | 5' | -47.1 | NC_002166.1 | + | 35682 | 0.67 | 0.946764 |
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Target: 5'- -cUGGCGaagAAGgggaagCCAauacuACAUCAGCg -3' miRNA: 3'- aaACCGCa--UUCa-----GGUguu--UGUAGUCG- -5' |
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| 8432 | 5' | -47.1 | NC_002166.1 | + | 21639 | 0.68 | 0.916913 |
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Target: 5'- --aGGCGUGAGUCacugAUAGACAgaauuaaaGGCg -3' miRNA: 3'- aaaCCGCAUUCAGg---UGUUUGUag------UCG- -5' |
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| 8432 | 5' | -47.1 | NC_002166.1 | + | 6670 | 0.69 | 0.887062 |
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Target: 5'- -cUGGCGUuuucuuGAGUUUgguacugaGCAcACAUCAGCg -3' miRNA: 3'- aaACCGCA------UUCAGG--------UGUuUGUAGUCG- -5' |
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| 8432 | 5' | -47.1 | NC_002166.1 | + | 35876 | 0.69 | 0.870224 |
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Target: 5'- --cGGCGgcGGgagUCugGAACGUCuGCg -3' miRNA: 3'- aaaCCGCauUCa--GGugUUUGUAGuCG- -5' |
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| 8432 | 5' | -47.1 | NC_002166.1 | + | 276 | 0.73 | 0.688633 |
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Target: 5'- --cGGCGgcuagaAGGUCgGCAGGCGUcCAGCu -3' miRNA: 3'- aaaCCGCa-----UUCAGgUGUUUGUA-GUCG- -5' |
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| 8432 | 5' | -47.1 | NC_002166.1 | + | 29300 | 1.1 | 0.003666 |
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Target: 5'- uUUUGGCGUAAGUCCACAAACAUCAGCc -3' miRNA: 3'- -AAACCGCAUUCAGGUGUUUGUAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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