Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8437 | 3' | -56.4 | NC_002166.1 | + | 15961 | 0.67 | 0.526865 |
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Target: 5'- -aGCG-UGAuccgcCGGGCAACCagUCgCCGCCu -3' miRNA: 3'- uaCGCuGCUu----GCCUGUUGG--AG-GGCGG- -5' |
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| 8437 | 3' | -56.4 | NC_002166.1 | + | 10688 | 0.67 | 0.526865 |
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Target: 5'- -aGCGGC--GCGGGucaguggcauaaUAGCCUCCgGCCc -3' miRNA: 3'- uaCGCUGcuUGCCU------------GUUGGAGGgCGG- -5' |
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| 8437 | 3' | -56.4 | NC_002166.1 | + | 26346 | 0.68 | 0.474452 |
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Target: 5'- cGUGCGGguaGAaaGCGGACAugacGCCgcuaCCCGCg -3' miRNA: 3'- -UACGCUg--CU--UGCCUGU----UGGa---GGGCGg -5' |
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| 8437 | 3' | -56.4 | NC_002166.1 | + | 5341 | 0.68 | 0.454221 |
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Target: 5'- gGUGCGAUGAACcagcauggaaGGuuGCGGCUgauauUCCUGCCg -3' miRNA: 3'- -UACGCUGCUUG----------CC--UGUUGG-----AGGGCGG- -5' |
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| 8437 | 3' | -56.4 | NC_002166.1 | + | 1732 | 0.71 | 0.311456 |
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Target: 5'- gAUGcCGAUGccauUGGGCugAGCCUCCCGCUg -3' miRNA: 3'- -UAC-GCUGCuu--GCCUG--UUGGAGGGCGG- -5' |
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| 8437 | 3' | -56.4 | NC_002166.1 | + | 37160 | 0.71 | 0.291742 |
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Target: 5'- uAUGC-ACGGgcaaaACGGcaugagguuguuagcGCAACCUCCUGCCa -3' miRNA: 3'- -UACGcUGCU-----UGCC---------------UGUUGGAGGGCGG- -5' |
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| 8437 | 3' | -56.4 | NC_002166.1 | + | 19065 | 0.72 | 0.267453 |
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Target: 5'- -gGC-ACGAACuGGACGGCa-CCCGCCa -3' miRNA: 3'- uaCGcUGCUUG-CCUGUUGgaGGGCGG- -5' |
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| 8437 | 3' | -56.4 | NC_002166.1 | + | 17164 | 0.72 | 0.267453 |
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Target: 5'- -gGCGACGGugaACGGGaauACCUC-CGCCa -3' miRNA: 3'- uaCGCUGCU---UGCCUgu-UGGAGgGCGG- -5' |
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| 8437 | 3' | -56.4 | NC_002166.1 | + | 3207 | 1.1 | 0.000425 |
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Target: 5'- gAUGCGACGAACGGACAACCUCCCGCCa -3' miRNA: 3'- -UACGCUGCUUGCCUGUUGGAGGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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