miRNA: 3'- -UGAC--CUAGACAUGGcaUCCUUAGCAA- -5'
miRNA: 3'- -UGACCUAgaCAUGGCAUCCUU---------AGCAa -5'
miRNA: 3'- -UGACCUAGACAUGGCAUCCUUAGCAA- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 844 | 3' | -50.1 | NC_000898.1 | + | 146182 | 0.72 | 0.884838 |
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Target: 5'- gACUGcaGAUCUGUACCacGGGAAUUGUUc -3' miRNA: 3'- -UGAC--CUAGACAUGGcaUCCUUAGCAA- -5' |
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| 844 | 3' | -50.1 | NC_000898.1 | + | 45203 | 0.77 | 0.669255 |
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Target: 5'- aGCUGGAUgaGUGCUGUAGGGAaguaacgauUCGUa -3' miRNA: 3'- -UGACCUAgaCAUGGCAUCCUU---------AGCAa -5' |
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| 844 | 3' | -50.1 | NC_000898.1 | + | 116111 | 1.05 | 0.018075 |
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Target: 5'- gACUGGAUCUGUACCGUAGGAAUCGUUc -3' miRNA: 3'- -UGACCUAGACAUGGCAUCCUUAGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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