miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8442 5' -55.1 NC_002166.1 + 36705 0.66 0.6281
Target:  5'- aCAGCGCgauaUGCGGCGCuugcuGGCauCCUGg -3'
miRNA:   3'- gGUUGUG----ACGUCGCGuu---CCGauGGGC- -5'
8442 5' -55.1 NC_002166.1 + 20905 0.66 0.605512
Target:  5'- gCCAACAUUccaugaacugcGUAGCcugucaGCGAGGCUAUaCCGg -3'
miRNA:   3'- -GGUUGUGA-----------CGUCG------CGUUCCGAUG-GGC- -5'
8442 5' -55.1 NC_002166.1 + 27330 0.67 0.583021
Target:  5'- cCCuGCGCaGC-GCGCAGGGUcaGCCUGa -3'
miRNA:   3'- -GGuUGUGaCGuCGCGUUCCGa-UGGGC- -5'
8442 5' -55.1 NC_002166.1 + 15886 0.68 0.524433
Target:  5'- cUCGAUACUGCuGCGCGuAGaGCUcgucagauucaaccACCCa -3'
miRNA:   3'- -GGUUGUGACGuCGCGU-UC-CGA--------------UGGGc -5'
8442 5' -55.1 NC_002166.1 + 16043 0.68 0.506097
Target:  5'- aCGAUAUucgcagGCGGCGacuGGUUGCCCGg -3'
miRNA:   3'- gGUUGUGa-----CGUCGCguuCCGAUGGGC- -5'
8442 5' -55.1 NC_002166.1 + 39187 0.69 0.443805
Target:  5'- cCCAcuugGCACUGaucaucccaaaUAGUGCGGGGCgacgACCUGg -3'
miRNA:   3'- -GGU----UGUGAC-----------GUCGCGUUCCGa---UGGGC- -5'
8442 5' -55.1 NC_002166.1 + 15387 0.71 0.333496
Target:  5'- aCCAGCGCUGUGGUGUAgcggguuuucguGGuGCUGCuuGa -3'
miRNA:   3'- -GGUUGUGACGUCGCGU------------UC-CGAUGggC- -5'
8442 5' -55.1 NC_002166.1 + 2087 0.73 0.271875
Target:  5'- aCCAAUaacgGCUGguGgGCAAGGCUGcaguCCUGg -3'
miRNA:   3'- -GGUUG----UGACguCgCGUUCCGAU----GGGC- -5'
8442 5' -55.1 NC_002166.1 + 13573 0.75 0.18645
Target:  5'- gCCuuGCGCUGCAGUGUu-GGUUACCCc -3'
miRNA:   3'- -GGu-UGUGACGUCGCGuuCCGAUGGGc -5'
8442 5' -55.1 NC_002166.1 + 13513 1.12 0.000403
Target:  5'- aCCAACACUGCAGCGCAAGGCUACCCGg -3'
miRNA:   3'- -GGUUGUGACGUCGCGUUCCGAUGGGC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.