miRNA: 3'- cuACAACU--AAU-GUCGAUGuGCG-C-GCG- -5'
miRNA: 3'- -CUACAa----CUAA--UGUCGAUGU----GCGCGcg -5'
miRNA: 3'- cuacaaCUAAUGUCGA------UGUgCGCgCG- -5'
miRNA: 3'- -CUACa--ACUAAUG---UCGAUGuGCGCG-CG- -5'
miRNA: 3'- -CUACAACUAAUGUCGAUGUGCGCGCG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8444 | 3' | -48.4 | NC_002166.1 | + | 38789 | 0.67 | 0.921892 |
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Target: 5'- -uUGUUGAacUUGuCAGCUGC-CGCaGuCGCg -3' miRNA: 3'- cuACAACU--AAU-GUCGAUGuGCG-C-GCG- -5' |
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| 8444 | 3' | -48.4 | NC_002166.1 | + | 11308 | 0.68 | 0.877599 |
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Target: 5'- uGAUGUgcugcGAUUucACAGCUACAgucuUGCGCa- -3' miRNA: 3'- -CUACAa----CUAA--UGUCGAUGU----GCGCGcg -5' |
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| 8444 | 3' | -48.4 | NC_002166.1 | + | 27321 | 0.69 | 0.84211 |
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Target: 5'- -----gGAUUACAGCUgugucaGCAgGCGgGCa -3' miRNA: 3'- cuacaaCUAAUGUCGA------UGUgCGCgCG- -5' |
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| 8444 | 3' | -48.4 | NC_002166.1 | + | 7052 | 0.7 | 0.770605 |
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Target: 5'- gGAUGaagUGAUUGCcguGGCUAC-UGCGCuGCg -3' miRNA: 3'- -CUACa--ACUAAUG---UCGAUGuGCGCG-CG- -5' |
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| 8444 | 3' | -48.4 | NC_002166.1 | + | 15252 | 1.12 | 0.002022 |
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Target: 5'- aGAUGUUGAUUACAGCUACACGCGCGCc -3' miRNA: 3'- -CUACAACUAAUGUCGAUGUGCGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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