Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8446 | 3' | -40.4 | NC_002166.1 | + | 19901 | 0.67 | 0.999951 |
Target: 5'- aUCCUuguaaacaaaaUUGACAAGcuca-AGCGCAAGu -3' miRNA: 3'- -AGGA-----------AACUGUUUuuuaaUCGCGUUCu -5' |
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8446 | 3' | -40.4 | NC_002166.1 | + | 19295 | 0.67 | 0.999931 |
Target: 5'- -----cGACGAGAAuAUUGGCGCGugGGAg -3' miRNA: 3'- aggaaaCUGUUUUU-UAAUCGCGU--UCU- -5' |
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8446 | 3' | -40.4 | NC_002166.1 | + | 10631 | 0.68 | 0.999824 |
Target: 5'- cCCUUUGGCAAAGGcaaacAGUGUugGAGAg -3' miRNA: 3'- aGGAAACUGUUUUUuaa--UCGCG--UUCU- -5' |
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8446 | 3' | -40.4 | NC_002166.1 | + | 36047 | 0.71 | 0.996576 |
Target: 5'- aCCgaagUGGCGAGGAugcuuuccuGCGCAAGAg -3' miRNA: 3'- aGGaa--ACUGUUUUUuaau-----CGCGUUCU- -5' |
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8446 | 3' | -40.4 | NC_002166.1 | + | 20527 | 0.87 | 0.478596 |
Target: 5'- aCCUgaGACAGAGcAUUAGCGCAAGGu -3' miRNA: 3'- aGGAaaCUGUUUUuUAAUCGCGUUCU- -5' |
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8446 | 3' | -40.4 | NC_002166.1 | + | 20423 | 1.13 | 0.016142 |
Target: 5'- cUCCUUUGACAAAAAAUUAGCGCAAGAa -3' miRNA: 3'- -AGGAAACUGUUUUUUAAUCGCGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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