Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8447 | 3' | -57.5 | NC_002166.1 | + | 8650 | 0.66 | 0.466956 |
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Target: 5'- aGCUGCAgCGGCAgcGGCAU-CCu-CCUUUa -3' miRNA: 3'- -CGACGU-GCCGU--CCGUAaGGucGGAGA- -5' |
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| 8447 | 3' | -57.5 | NC_002166.1 | + | 33949 | 0.66 | 0.446685 |
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Target: 5'- uGCUGgAUGGCuugaauGGCGUUCUgcugauggauggGGCUUCUu -3' miRNA: 3'- -CGACgUGCCGu-----CCGUAAGG------------UCGGAGA- -5' |
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| 8447 | 3' | -57.5 | NC_002166.1 | + | 32327 | 0.67 | 0.407671 |
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Target: 5'- cGCguuCACGGCGGcGCAUUUCAGCagaCUg -3' miRNA: 3'- -CGac-GUGCCGUC-CGUAAGGUCGga-GA- -5' |
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| 8447 | 3' | -57.5 | NC_002166.1 | + | 40061 | 0.67 | 0.398255 |
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Target: 5'- -aUGCACGGC-GGCGUUCUGG-UUCa -3' miRNA: 3'- cgACGUGCCGuCCGUAAGGUCgGAGa -5' |
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| 8447 | 3' | -57.5 | NC_002166.1 | + | 4347 | 0.68 | 0.336385 |
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Target: 5'- cGCUGauuCGGCuGGCucacugaUCCAGCCUa- -3' miRNA: 3'- -CGACgu-GCCGuCCGua-----AGGUCGGAga -5' |
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| 8447 | 3' | -57.5 | NC_002166.1 | + | 265 | 0.69 | 0.328139 |
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Target: 5'- --aGguCGGCAGGCGU-CCAGCUg-- -3' miRNA: 3'- cgaCguGCCGUCCGUAaGGUCGGaga -5' |
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| 8447 | 3' | -57.5 | NC_002166.1 | + | 17900 | 0.69 | 0.289168 |
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Target: 5'- cGCUGCAUcuucuguaGGUcuGGGUAauuaUCCGGCCUCa -3' miRNA: 3'- -CGACGUG--------CCG--UCCGUa---AGGUCGGAGa -5' |
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| 8447 | 3' | -57.5 | NC_002166.1 | + | 23348 | 1.09 | 0.000351 |
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Target: 5'- gGCUGCACGGCAGGCAUUCCAGCCUCUc -3' miRNA: 3'- -CGACGUGCCGUCCGUAAGGUCGGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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