Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8448 | 3' | -52.8 | NC_002166.1 | + | 15254 | 0.66 | 0.803523 |
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Target: 5'- cCCAGUACgUCaUGccACGGAAUaugGCCGc -3' miRNA: 3'- -GGUCGUGgAG-GCuaUGCCUUAa--CGGC- -5' |
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| 8448 | 3' | -52.8 | NC_002166.1 | + | 7702 | 0.66 | 0.793569 |
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Target: 5'- uCCAGCG-CUCCGGcucugcugUAUGGG--UGCCa -3' miRNA: 3'- -GGUCGUgGAGGCU--------AUGCCUuaACGGc -5' |
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| 8448 | 3' | -52.8 | NC_002166.1 | + | 4831 | 0.67 | 0.742438 |
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Target: 5'- uUCAGCGCUUCCGGUaucguccugaacccGCGcGAcuggcacaacaucgcGUUGCUGa -3' miRNA: 3'- -GGUCGUGGAGGCUA--------------UGC-CU---------------UAACGGC- -5' |
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| 8448 | 3' | -52.8 | NC_002166.1 | + | 24859 | 0.69 | 0.607024 |
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Target: 5'- uCCGGCAaUUCCGuauCGGAggUGCUGg -3' miRNA: 3'- -GGUCGUgGAGGCuauGCCUuaACGGC- -5' |
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| 8448 | 3' | -52.8 | NC_002166.1 | + | 6611 | 0.69 | 0.607024 |
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Target: 5'- cCUGGCACCgcccauuaccCCGAcACGGAAU-GCCa -3' miRNA: 3'- -GGUCGUGGa---------GGCUaUGCCUUAaCGGc -5' |
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| 8448 | 3' | -52.8 | NC_002166.1 | + | 24899 | 0.7 | 0.539859 |
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Target: 5'- gCCuGCGCCUCUguauGGUGCGGAugacgcgcGCCGc -3' miRNA: 3'- -GGuCGUGGAGG----CUAUGCCUuaa-----CGGC- -5' |
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| 8448 | 3' | -52.8 | NC_002166.1 | + | 14053 | 0.77 | 0.238155 |
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Target: 5'- aCUGGCGCaCUCUGGUACGGAGggaugGCUGa -3' miRNA: 3'- -GGUCGUG-GAGGCUAUGCCUUaa---CGGC- -5' |
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| 8448 | 3' | -52.8 | NC_002166.1 | + | 24792 | 1.12 | 0.000877 |
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Target: 5'- uCCAGCACCUCCGAUACGGAAUUGCCGg -3' miRNA: 3'- -GGUCGUGGAGGCUAUGCCUUAACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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