Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8452 | 3' | -55.3 | NC_002167.1 | + | 9313 | 0.66 | 0.565044 |
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Target: 5'- uACGGCuGCcGGAGcuUUGGCUGCGAUa -3' miRNA: 3'- -UGCCGcCGuCCUUauAGUCGGCGUUG- -5' |
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| 8452 | 3' | -55.3 | NC_002167.1 | + | 8109 | 0.67 | 0.520654 |
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Target: 5'- -aGGCGGCAcu--UA-CAGCUGCGACa -3' miRNA: 3'- ugCCGCCGUccuuAUaGUCGGCGUUG- -5' |
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| 8452 | 3' | -55.3 | NC_002167.1 | + | 9520 | 0.68 | 0.453793 |
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Target: 5'- gGCGGCGGCcuugcguugugauuGGGuagcagauUCAGCCcuuGCAGCu -3' miRNA: 3'- -UGCCGCCG--------------UCCuuau----AGUCGG---CGUUG- -5' |
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| 8452 | 3' | -55.3 | NC_002167.1 | + | 1434 | 0.68 | 0.446649 |
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Target: 5'- cGCGG-GGCuGGAucUGUCAGCCaGgAACg -3' miRNA: 3'- -UGCCgCCGuCCUu-AUAGUCGG-CgUUG- -5' |
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| 8452 | 3' | -55.3 | NC_002167.1 | + | 23187 | 0.69 | 0.378876 |
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Target: 5'- aACGGCGuuGCGGGAGUGUCGuucuGCUGgAAUc -3' miRNA: 3'- -UGCCGC--CGUCCUUAUAGU----CGGCgUUG- -5' |
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| 8452 | 3' | -55.3 | NC_002167.1 | + | 34863 | 0.71 | 0.318353 |
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Target: 5'- cGCGGCGGCGGGAGUcuggaaCGuCUGCGAUu -3' miRNA: 3'- -UGCCGCCGUCCUUAua----GUcGGCGUUG- -5' |
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| 8452 | 3' | -55.3 | NC_002167.1 | + | 6826 | 0.73 | 0.226492 |
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Target: 5'- gUGGaCGGCAGGGAggaaauaaggaucucGUCAGCUGCGAUu -3' miRNA: 3'- uGCC-GCCGUCCUUa--------------UAGUCGGCGUUG- -5' |
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| 8452 | 3' | -55.3 | NC_002167.1 | + | 5429 | 1.08 | 0.000622 |
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Target: 5'- aACGGCGGCAGGAAUAUCAGCCGCAACc -3' miRNA: 3'- -UGCCGCCGUCCUUAUAGUCGGCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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