Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8455 | 3' | -48.1 | NC_002167.1 | + | 10765 | 0.66 | 0.951431 |
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Target: 5'- cUGGGCUacGGGCAGGGaacCAGGUUCcgUGa -3' miRNA: 3'- aGCCCGA--CCUGUCUU---GUUUAAGuuGC- -5' |
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| 8455 | 3' | -48.1 | NC_002167.1 | + | 17749 | 0.67 | 0.929986 |
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Target: 5'- aUCGGGCaGGuuCAGAcCAGcgUUAAUGa -3' miRNA: 3'- -AGCCCGaCCu-GUCUuGUUuaAGUUGC- -5' |
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| 8455 | 3' | -48.1 | NC_002167.1 | + | 37687 | 0.68 | 0.891405 |
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Target: 5'- aCGauGGCUGGuuugacgcgacugcgGCAGccGACAAGUUCAACa -3' miRNA: 3'- aGC--CCGACC---------------UGUC--UUGUUUAAGUUGc -5' |
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| 8455 | 3' | -48.1 | NC_002167.1 | + | 1437 | 0.69 | 0.845202 |
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Target: 5'- -gGGGCUGGAUcugucagccaGGAACGAc-UUAACGg -3' miRNA: 3'- agCCCGACCUG----------UCUUGUUuaAGUUGC- -5' |
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| 8455 | 3' | -48.1 | NC_002167.1 | + | 30567 | 0.72 | 0.70691 |
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Target: 5'- aCGGGCUGGACG--GCAGAUUUu--- -3' miRNA: 3'- aGCCCGACCUGUcuUGUUUAAGuugc -5' |
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| 8455 | 3' | -48.1 | NC_002167.1 | + | 16541 | 0.81 | 0.250214 |
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Target: 5'- gUCGGGCUGGACGGAAac-AUUCcGCa -3' miRNA: 3'- -AGCCCGACCUGUCUUguuUAAGuUGc -5' |
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| 8455 | 3' | -48.1 | NC_002167.1 | + | 12574 | 1.12 | 0.002444 |
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Target: 5'- aUCGGGCUGGACAGAACAAAUUCAACGg -3' miRNA: 3'- -AGCCCGACCUGUCUUGUUUAAGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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