Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8458 | 5' | -53.8 | NC_002167.1 | + | 15642 | 0.66 | 0.695572 |
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Target: 5'- gAGCGcAGCCaCCGuaUCGAUUUACC-GCAGg -3' miRNA: 3'- -UCGU-UUGG-GGC--AGUUAGGUGGuCGUC- -5' |
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| 8458 | 5' | -53.8 | NC_002167.1 | + | 12540 | 0.66 | 0.661402 |
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Target: 5'- cGGCAAGCucgccaguuuuCCCGUCGAUauaccagaCCugCAGCu- -3' miRNA: 3'- -UCGUUUG-----------GGGCAGUUA--------GGugGUCGuc -5' |
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| 8458 | 5' | -53.8 | NC_002167.1 | + | 1823 | 0.67 | 0.626963 |
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Target: 5'- --aAAGCCCUGgccgAAUCgACCAGCGGg -3' miRNA: 3'- ucgUUUGGGGCag--UUAGgUGGUCGUC- -5' |
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| 8458 | 5' | -53.8 | NC_002167.1 | + | 1777 | 0.67 | 0.604009 |
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Target: 5'- cGGCAuccuUCCUgaacugGUCAAUgCGCCAGCGGu -3' miRNA: 3'- -UCGUuu--GGGG------CAGUUAgGUGGUCGUC- -5' |
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| 8458 | 5' | -53.8 | NC_002167.1 | + | 30857 | 0.69 | 0.514001 |
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Target: 5'- gAGCAuuUCaCGUCAAUCCugaugACCAGCAa -3' miRNA: 3'- -UCGUuuGGgGCAGUUAGG-----UGGUCGUc -5' |
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| 8458 | 5' | -53.8 | NC_002167.1 | + | 38066 | 0.69 | 0.47102 |
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Target: 5'- gGGCu-GCCCCGUC-AUCgGCCAGg-- -3' miRNA: 3'- -UCGuuUGGGGCAGuUAGgUGGUCguc -5' |
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| 8458 | 5' | -53.8 | NC_002167.1 | + | 14243 | 1.08 | 0.00096 |
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Target: 5'- cAGCAAACCCCGUCAAUCCACCAGCAGc -3' miRNA: 3'- -UCGUUUGGGGCAGUUAGGUGGUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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