Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8459 | 3' | -53.4 | NC_002167.1 | + | 3227 | 0.66 | 0.704973 |
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Target: 5'- uGCCGCCAuuaccgggugGCGAUG-UAGCGaUGCGa-- -3' miRNA: 3'- -UGGCGGU----------UGUUGCaAUCGC-GCGCaua -5' |
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| 8459 | 3' | -53.4 | NC_002167.1 | + | 14994 | 0.67 | 0.647731 |
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Target: 5'- cACCGUCGGCAAU--UAGUGCGCcaGUGc -3' miRNA: 3'- -UGGCGGUUGUUGcaAUCGCGCG--CAUa -5' |
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| 8459 | 3' | -53.4 | NC_002167.1 | + | 28094 | 0.7 | 0.457664 |
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Target: 5'- aACCaaGCCAGCuucauCGUUGGCG-GCGUGUu -3' miRNA: 3'- -UGG--CGGUUGuu---GCAAUCGCgCGCAUA- -5' |
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| 8459 | 3' | -53.4 | NC_002167.1 | + | 30715 | 0.71 | 0.388083 |
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Target: 5'- aACCGCCAGCGACGUucUGGCaaCGCu--- -3' miRNA: 3'- -UGGCGGUUGUUGCA--AUCGc-GCGcaua -5' |
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| 8459 | 3' | -53.4 | NC_002167.1 | + | 32282 | 0.73 | 0.286083 |
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Target: 5'- gGCCGCCAcagcgGCGugGUUAGCGCa-GUAg -3' miRNA: 3'- -UGGCGGU-----UGUugCAAUCGCGcgCAUa -5' |
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| 8459 | 3' | -53.4 | NC_002167.1 | + | 16337 | 1.06 | 0.001385 |
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Target: 5'- gACCGCCAACAACGUUAGCGCGCGUAUa -3' miRNA: 3'- -UGGCGGUUGUUGCAAUCGCGCGCAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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