Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8459 | 5' | -55 | NC_002167.1 | + | 38295 | 0.66 | 0.624424 |
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Target: 5'- uCGAag-CGCGuuGCAUGUCGGCauucCAGGu -3' miRNA: 3'- -GUUguaGCGCgcCGUACAGUCG----GUCC- -5' |
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| 8459 | 5' | -55 | NC_002167.1 | + | 16430 | 0.67 | 0.568187 |
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Target: 5'- --cCAUCGCGuCGGCAUaagcGUUAcCCGGGu -3' miRNA: 3'- guuGUAGCGC-GCCGUA----CAGUcGGUCC- -5' |
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| 8459 | 5' | -55 | NC_002167.1 | + | 34344 | 0.68 | 0.513301 |
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Target: 5'- gCAAagaagCGCGCGGCAgagaauGCCAGGu -3' miRNA: 3'- -GUUgua--GCGCGCCGUacagu-CGGUCC- -5' |
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| 8459 | 5' | -55 | NC_002167.1 | + | 18012 | 0.69 | 0.468941 |
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Target: 5'- aCAACGUuuuaCGCGCGGCuaccugcGCCAGGu -3' miRNA: 3'- -GUUGUA----GCGCGCCGuacagu-CGGUCC- -5' |
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| 8459 | 5' | -55 | NC_002167.1 | + | 17870 | 0.69 | 0.450536 |
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Target: 5'- aCAGCAUCaGCGUgucuauggacGGCAUGaCGGgCGGGg -3' miRNA: 3'- -GUUGUAG-CGCG----------CCGUACaGUCgGUCC- -5' |
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| 8459 | 5' | -55 | NC_002167.1 | + | 5585 | 0.69 | 0.440484 |
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Target: 5'- aCGACuUCGCGCGaucCAUcugGUCAGCCGGu -3' miRNA: 3'- -GUUGuAGCGCGCc--GUA---CAGUCGGUCc -5' |
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| 8459 | 5' | -55 | NC_002167.1 | + | 18420 | 0.72 | 0.291299 |
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Target: 5'- --cCGUgGUGaCGGCGaacggGUCAGCCAGGa -3' miRNA: 3'- guuGUAgCGC-GCCGUa----CAGUCGGUCC- -5' |
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| 8459 | 5' | -55 | NC_002167.1 | + | 1427 | 0.77 | 0.143431 |
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Target: 5'- aGugGUaCGCGgGGCuggaucUGUCAGCCAGGa -3' miRNA: 3'- gUugUA-GCGCgCCGu-----ACAGUCGGUCC- -5' |
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| 8459 | 5' | -55 | NC_002167.1 | + | 16302 | 1.11 | 0.000509 |
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Target: 5'- uCAACAUCGCGCGGCAUGUCAGCCAGGg -3' miRNA: 3'- -GUUGUAGCGCGCCGUACAGUCGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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