Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
846 | 5' | -55.8 | NC_000898.1 | + | 157629 | 0.66 | 0.940263 |
Target: 5'- gGGGGACgggggUGUGGcg-CCGacuCUGCCGCg -3' miRNA: 3'- -CCCUUG-----GCACCuuaGGUgu-GGCGGCG- -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 3286 | 0.66 | 0.935546 |
Target: 5'- cGGAcgugcgagACCGagcGGGcUCCAU-CCGCCGCu -3' miRNA: 3'- cCCU--------UGGCa--CCUuAGGUGuGGCGGCG- -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 156453 | 0.66 | 0.935546 |
Target: 5'- cGGAcgugcgagACCGagcGGGcUCCAU-CCGCCGCu -3' miRNA: 3'- cCCU--------UGGCa--CCUuAGGUGuGGCGGCG- -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 46693 | 0.66 | 0.92489 |
Target: 5'- aGGGuuccguuaaauGGCCGUGaGAGUUCAUGCugugugaCGCCGUa -3' miRNA: 3'- -CCC-----------UUGGCAC-CUUAGGUGUG-------GCGGCG- -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 9972 | 0.67 | 0.920012 |
Target: 5'- cGGGAcauCUGUGGA--CCAUGCaGCUGCc -3' miRNA: 3'- -CCCUu--GGCACCUuaGGUGUGgCGGCG- -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 147008 | 0.67 | 0.920012 |
Target: 5'- ---uGCCGUguGGAAUCCGCAgCCGUCa- -3' miRNA: 3'- cccuUGGCA--CCUUAGGUGU-GGCGGcg -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 10059 | 0.67 | 0.896086 |
Target: 5'- gGGGAGCCGUGGugcgCCuguCCGauaGCu -3' miRNA: 3'- -CCCUUGGCACCuua-GGuguGGCgg-CG- -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 5266 | 0.67 | 0.889542 |
Target: 5'- cGGGcucGCgCGUGGGccaAUCgCGCcCCGUCGCg -3' miRNA: 3'- -CCCu--UG-GCACCU---UAG-GUGuGGCGGCG- -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 158433 | 0.67 | 0.889542 |
Target: 5'- cGGGcucGCgCGUGGGccaAUCgCGCcCCGUCGCg -3' miRNA: 3'- -CCCu--UG-GCACCU---UAG-GUGuGGCGGCG- -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 36912 | 0.7 | 0.786491 |
Target: 5'- cGGGAAgaGgaUGGAucgCCcucggACGCCGCCGCc -3' miRNA: 3'- -CCCUUggC--ACCUua-GG-----UGUGGCGGCG- -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 3835 | 0.72 | 0.688106 |
Target: 5'- uGGGAggagcguuGCCGUGGAGgcguucgggggacuUCC-CGCCGaCGCg -3' miRNA: 3'- -CCCU--------UGGCACCUU--------------AGGuGUGGCgGCG- -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 157003 | 0.72 | 0.688106 |
Target: 5'- uGGGAggagcguuGCCGUGGAGgcguucgggggacuUCC-CGCCGaCGCg -3' miRNA: 3'- -CCCU--------UGGCACCUU--------------AGGuGUGGCgGCG- -5' |
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846 | 5' | -55.8 | NC_000898.1 | + | 152246 | 1.13 | 0.002063 |
Target: 5'- cGGGAACCGUGGAAUCCACACCGCCGCc -3' miRNA: 3'- -CCCUUGGCACCUUAGGUGUGGCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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