Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8460 | 5' | -55.3 | NC_002167.1 | + | 33481 | 0.66 | 0.657034 |
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Target: 5'- uCGAgCGggcuUUAACGuGCGCUaacUGCGGUCa -3' miRNA: 3'- -GCUgGC----AAUUGC-CGCGAcu-ACGCCGG- -5' |
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| 8460 | 5' | -55.3 | NC_002167.1 | + | 16392 | 0.66 | 0.645876 |
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Target: 5'- -aACCGguauuugAGCaGGCGCUGGUggcGCGGUa -3' miRNA: 3'- gcUGGCaa-----UUG-CCGCGACUA---CGCCGg -5' |
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| 8460 | 5' | -55.3 | NC_002167.1 | + | 2234 | 0.67 | 0.601219 |
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Target: 5'- uCGACUGUg--UGGCGCUGc-GUGaGCCu -3' miRNA: 3'- -GCUGGCAauuGCCGCGACuaCGC-CGG- -5' |
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| 8460 | 5' | -55.3 | NC_002167.1 | + | 786 | 0.67 | 0.546068 |
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Target: 5'- aGAUUGUcAGCGGUGCacucagccggGAacagGCGGCCa -3' miRNA: 3'- gCUGGCAaUUGCCGCGa---------CUa---CGCCGG- -5' |
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| 8460 | 5' | -55.3 | NC_002167.1 | + | 19386 | 0.68 | 0.535214 |
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Target: 5'- uGACgGUUAcuccagcuGCuuccccuGCGCUGA-GCGGCCa -3' miRNA: 3'- gCUGgCAAU--------UGc------CGCGACUaCGCCGG- -5' |
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| 8460 | 5' | -55.3 | NC_002167.1 | + | 11572 | 0.68 | 0.524438 |
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Target: 5'- cCGGCC-UUGAgcaauuUGGUGCUGAUGuCGGUa -3' miRNA: 3'- -GCUGGcAAUU------GCCGCGACUAC-GCCGg -5' |
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| 8460 | 5' | -55.3 | NC_002167.1 | + | 1460 | 0.69 | 0.451681 |
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Target: 5'- aCGACU--UAACGGCGCUGGUuaucGCuGGUg -3' miRNA: 3'- -GCUGGcaAUUGCCGCGACUA----CG-CCGg -5' |
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| 8460 | 5' | -55.3 | NC_002167.1 | + | 23137 | 0.73 | 0.265107 |
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Target: 5'- aGGCCaaGUgcGCGGCGCUGGUaGCGGa- -3' miRNA: 3'- gCUGG--CAauUGCCGCGACUA-CGCCgg -5' |
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| 8460 | 5' | -55.3 | NC_002167.1 | + | 16687 | 1.13 | 0.000398 |
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Target: 5'- gCGACCGUUAACGGCGCUGAUGCGGCCg -3' miRNA: 3'- -GCUGGCAAUUGCCGCGACUACGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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