Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8461 | 3' | -50.3 | NC_002167.1 | + | 17330 | 0.66 | 0.895214 |
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Target: 5'- uCGAGCUGAaCugGggAUUC-CcGGCg -3' miRNA: 3'- uGCUCGACUaGugCuaUAAGcGuCCG- -5' |
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| 8461 | 3' | -50.3 | NC_002167.1 | + | 36900 | 0.66 | 0.895214 |
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Target: 5'- -gGGGCUGGUUAUGGccaGUUCGagcaUAGGCu -3' miRNA: 3'- ugCUCGACUAGUGCUa--UAAGC----GUCCG- -5' |
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| 8461 | 3' | -50.3 | NC_002167.1 | + | 4482 | 0.68 | 0.785808 |
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Target: 5'- cUGAGCcagcaGAUCACGAaugGUaagacggCGCAGGCc -3' miRNA: 3'- uGCUCGa----CUAGUGCUa--UAa------GCGUCCG- -5' |
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| 8461 | 3' | -50.3 | NC_002167.1 | + | 448 | 0.69 | 0.742576 |
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Target: 5'- -gGAGUgGAUCugGAgg-UCGCGGcGCu -3' miRNA: 3'- ugCUCGaCUAGugCUauaAGCGUC-CG- -5' |
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| 8461 | 3' | -50.3 | NC_002167.1 | + | 5289 | 0.84 | 0.110943 |
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Target: 5'- gACGAGCUGAUCACGcUGUaCGCccAGGCc -3' miRNA: 3'- -UGCUCGACUAGUGCuAUAaGCG--UCCG- -5' |
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| 8461 | 3' | -50.3 | NC_002167.1 | + | 17057 | 1.13 | 0.001102 |
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Target: 5'- aACGAGCUGAUCACGAUAUUCGCAGGCg -3' miRNA: 3'- -UGCUCGACUAGUGCUAUAAGCGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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